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J Acupunct Res > Volume 29(6); 2012 > Article
Published online December 17, 2012.
DOI: https://doi.org/10.13045/kamms.2012082   
Effects of Astragalus Membranaceus, Innamomum Cassia, Phellodendron Amurensis(BHH10) on MC3T3-E1 Cells Proliferation, Differntiation and Bone Mineralized Formation
Mi-lim Lee1, Jeong-eun Huh2, Dong-woo Nam1, Do-youn Choi1, Jae-dong Lee1
1Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Kyung-Hee University
2Oriental Medicine Research Center for Bone & Joint Disease, Kyung-Hee University
*Corresponding author.
Jae-dong Lee
Tel: +82-2-958-9208   Email: ljdacu@khmc.or.kr
Abstract
Objectives : BHH10 is traditional medicine herb used for enhancing body resistance against various diseases. The aim of this study was to identify BHH10 extract induces osteogenic activity in human osteoblast-like MC3T3-E1 cells.
Methods : MC3T3-E1, pre-osteoblast cell line, were treated with BHH10 of various concentrations(0.1 ㎍/mL, 1 ㎍/mL, 10 ㎍/mL). And then, the effect of BHH10 on osteoblast differentiation was examined by alkaline phosphatase(ALP) activity, von Kossa staining and RT-PCR for osteoblast differentiation markers such as osteocalcin(OCN), osteopontin(OPN).
Results : BHH10 had dose-dependent effect on the viability of osteoblastic cells, and dose-dependently increased alkaline phosphatase(ALP) activity. BHH10 markedly increased mRNA expression for OCN, OPN in MC3T3-E1 cells. Also, BHH10 significantly induced mineralization in the culture of MC3T3-E1 cells.
Conclusions : In conclusion, these results propose that BHH10 can play an important role in osteoblastic bone formation, osteogenesis, and may possibly lead to the development of bone-forming drugs.
Keywords: BHH10, ALP activity, OCN, OPN, mineralization
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