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J Acupunct Res > Volume 38(1); 2021 > Article
Published online February 22, 2021.
DOI: https://doi.org/10.13045/jar.2020.00332   
Single-dose and 4-week repeated dose Toxicity of Aconitum Sinomontanum Nakai Pharmacopuncture: An Experimental Study
Sang Ha Woo  , Jung Hee Lee  , Cho-in Lee  , Yun Kyu Lee  , Hyun-Jong Lee  , Jae Soo Kim 
Department of Acupuncture and Moxibustion medicine, College of Korean medicine, Daegu Hanni University, Daegu, Korea
*Corresponding author.
Jae Soo Kim
Email: jaice@daum.net
Received: October 6, 2020   Revised: November 11, 2020   Accepted: December 7, 2020
Abstract
Background
This study aimed to assess the toxicity of Aconitum sinomontanum Nakai (ASN) pharmacopuncture.

Methods

To investigate the toxicity of ASN pharmacopuncture, single and 4-week repeated dose toxicity experiments were conducted on BALB/c mice. In the single-dose toxicity experiment, mice were assigned 1 of 4 groups (5 males, 5 females per group). Then, 31.25, 62.5, and 125 mg/kg of ASN pharmacopuncture were administered to the mice in the experimental groups at acupoint ST36, while 0.2 mL of normal saline was administered to the control group at ST36. After a 4-week repeated dose regimen, the mice were assigned into 4 groups (5 males, 5 females per group). Then, 15.625, 31.25, and 62.5 mg/kg of ASN pharmacopuncture at ST36 were administered to the mice in the experimental groups, while 0.2 mL of normal saline was administered to the control group at ST36. Mortality, morbidity, general body and organ weight changes (after 4 weeks repeated dose), serum hematological and biochemical values, and histopathological changes in the liver and kidney were observed.

Results

In both single and 4-week repeated dose toxicity experiments, no deaths or symptoms occurred in any of the groups. There were no significant differences between groups in terms of body and organ weights, serum hematological and biochemical values, and specific organ histopathological changes.

Conclusion
ASN pharmacopuncture injection did not demonstrate significant toxicity in BALB/c mice compared with the control group, with a no-observed-adverse-effect level for a single dose of >125 mg/kg, and for 4 weeks repeated dose it was more than 62.5 mg/kg/day
Keywords: pharmacopuncture, toxicity, morbidity, body weight changes, hematologic test


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