The Effects of LR3 and SP6 Acupuncture on Renal Damage in Streptozotocin-induced Diabetic Mice

Article information

Acupunct. 2015;32(3):41-51
Department of Acupuncture & Moxibustion, Meridian & Acupoint, College of Oriental Medicine, Daegu Haany University
*Corresponding author: Department of Acupuncture & Moxibustion, Medicine, Daegu Oriental Hospital of Daegu Haany University, 136, Sincheondong-ro, Suseong-gu, Daegu, 42158, Republic of Korea, Tel : +82-53-770-2112, E-mail: jaice@daum.net
Received 2015 July 31; Revised 2015 September 02; Accepted 2015 September 09.

Abstract

Objectives:

This study was performed to investigate the effects of LR3 and SP6 acupuncture on renal damage in streptozotocin(STZ)-induced diabetic mice.

Methods:

ICR male mice were stabilized for a week and divided into four groups: a normal mice group(N), no-acupuncture diabetic mice group(Control), LR3 acupuncture diabetic mice group(LR3), and SP6 acupuncture diabetic mice group(SP6). Diabetes was experimentally induced by intraperitoneal injection of STZ(150 mg/kg) in citrate buffer(pH 4.5). For two weeks, LR3 and SP6 acupunctures were administered bilaterally at each point once a day. After two weeks, the animals’ weight was measured and they underwent a laparotomy. Serum glucose and blood urea nitrogen(BUN) were measured from the blood taken from the heart. We measured glucose, reactive oxygen species(ROS), peroxynitrite(ONOO) and thiobarbituric acid reactive substances(TBARS) in the kidney and compared expression levels of superoxide dismutases(SOD), glutathione peroxidase(GPx), nuclear factor-kappa B(NF-kB), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase(iNOS) and Interleukin-1 beta(IL-1β).

Results:

BUN significantly decreased in LR3, SP6 compared to the control group. LR3 showed significantly decreased glucose compared to the control group. LR3, SP6 significantly decreased in ROS and ONOO compared to the control group. LR3 significantly decreased in TBARS compared to the control group. SP6 significantly increased in expressions of SOD-1, catalase, and GPx compared to the control group. LR3, SP6 significantly decreased in COX-2 compared to the control group. SP6 significantly decreased in IL-1β compared to the control group.

Conclusions:

This study suggests that LR3 acupuncture may be effective in controlling glucose and lipid peroxidation and that SP6 acupuncture may have anti-oxidative and anti-inflammatory effects on renal damage in STZ-induced diabetic mice.

Fig. 1

The concentrations of glucose in the kidney

# : p<0.05 compared to normal group by ANOVA followed by Dunnett’s test.

* : p<0.05 compared to control group by ANOVA followed by Dunnett’s test.

Fig. 2

The concentrations of ROS, TBARS, ONOO production in the kidney

(A) reactive oxygen species(ROS). (B) thiobarbituric acid reactive substances(TBARS). (C) peroxynitrite(ONOO).

### : p<0.01 compared to normal group by ANOVA followed by Dunnett’s test.

* : p<0.05, **: p<0.01, *** : p<0.001 compared to control group by ANOVA followed by Dunnett’s test.

Fig. 3

The expression of antioxidant enzymes-related protein in the kidney

(A) superoxide dismutase(SOD). (B) catalase. (C) glutathion peroxidase(GPx).

# : p<0.05, ## : p<0.01 compared to normal group by ANOVA followed by Dunnett’s test. *p<0.05,

* : p<0.05, ** : p<0.01, *** : p<0.001 compared to control group by ANOVA followed by Dunnett’s test.

Fig. 4

The NF-κB p65 expressions in the kidney

# : p<0.05 compared to normal group by ANOVA followed by Dunnett’s test.

Fig. 5

The expressions inflammation-related protein in the kidney

(A) cyclooxygenase-2(COX-2). (B) inducible nitric oxide synthase(iNOS). (C) Interleukin-1 beta(IL-1β).

# : p<0.05, ## : p<0.01, ### : p<0.01 compared to normal group by ANOVA followed by Dunnett’s test.

** : p<0.01 compared to control group by ANOVA followed by Dunnett’s test.

Fig. 6

The effects and mechanisms of LR3 and SP6 acupuncture on renal protection against hyperglycemic-induced oxidative stress at the kidney

The Body Weight Change, Food and Water Intake

The Hematological Analyses

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Article information Continued

Fig. 1

The concentrations of glucose in the kidney

# : p<0.05 compared to normal group by ANOVA followed by Dunnett’s test.

* : p<0.05 compared to control group by ANOVA followed by Dunnett’s test.

Fig. 2

The concentrations of ROS, TBARS, ONOO production in the kidney

(A) reactive oxygen species(ROS). (B) thiobarbituric acid reactive substances(TBARS). (C) peroxynitrite(ONOO).

### : p<0.01 compared to normal group by ANOVA followed by Dunnett’s test.

* : p<0.05, **: p<0.01, *** : p<0.001 compared to control group by ANOVA followed by Dunnett’s test.

Fig. 3

The expression of antioxidant enzymes-related protein in the kidney

(A) superoxide dismutase(SOD). (B) catalase. (C) glutathion peroxidase(GPx).

# : p<0.05, ## : p<0.01 compared to normal group by ANOVA followed by Dunnett’s test. *p<0.05,

* : p<0.05, ** : p<0.01, *** : p<0.001 compared to control group by ANOVA followed by Dunnett’s test.

Fig. 4

The NF-κB p65 expressions in the kidney

# : p<0.05 compared to normal group by ANOVA followed by Dunnett’s test.

Fig. 5

The expressions inflammation-related protein in the kidney

(A) cyclooxygenase-2(COX-2). (B) inducible nitric oxide synthase(iNOS). (C) Interleukin-1 beta(IL-1β).

# : p<0.05, ## : p<0.01, ### : p<0.01 compared to normal group by ANOVA followed by Dunnett’s test.

** : p<0.01 compared to control group by ANOVA followed by Dunnett’s test.

Fig. 6

The effects and mechanisms of LR3 and SP6 acupuncture on renal protection against hyperglycemic-induced oxidative stress at the kidney

Table 1

The Body Weight Change, Food and Water Intake

Group Body weight change
(g / 14 days)
Food intake
(g / day)
Water intake
(ml / day)
Nornal 3.39±0.56 5.87±0.15 8.63±0.55
Control 0.89±0.32# 11.25±0.46## 5 0.04±1.62##
LR3 0.85±0.06 9.55±1.33 44.50±3.81
SP6 0.98±0.20 13.14±3.32 52.34±2.38

All values were showed mean±SD.

#

p<0.05,

##

p<0.01 compared to normal group by ANOVA followed by Dunnett’s test.

Table 2

The Hematological Analyses

Group glucose
(mg/dl)
BUN
(mg/dl)
Normal 94.1±0.7 27.2±1.0
Control 260.7±85.6# 93.9±0.8###
LR3 151.9±41.4 88.7±0.4***
SP6 289.8±5.2 86.2±0.6***

All values were showed mean±SD.

#

p<0.05,

###

p<0.001 compared to normal group by ANOVA followed by Dunnett’s test.

***

p<0.001 compared to control group by ANOVA followed by Dunnett’s test.