The Effect of Poria Cocos Pharmacopuncture at SP6(Sameumgyo) and ST36(Joksamni) on Chronic Stress Model(CSM) Rats

Article information

Acupunct. 2015;32(2):187-195
Department of Acupuncture and Moxibustion Medicine, College of Oriental Medicine, Dongshin University
*Corresponding author: Department of Acupuncture & Moxibustion Medicine, Mokpo Oriental Hospital of Dongshin University, 313, Baengnyeon-daero, Mokpo-si, Jeollanam-do, 530-822, Republic of Korea, Tel: +82-61-280-7903, E-mail: fire-sjc@hanmail.net
Received 2015 April 24; Revised 2015 May 18; Accepted 2015 May 27.

Abstract

Objectives:

The purpose of this study is evaluating the effect of Poria cocos pharmacopuncture (PCP) at SP6(Sameumgyo) and ST36(Joksamni) on chronic stress model(CSM) rats.

Methods:

The experimental rats were assigned to five groups: normal, CSM(control), PCP1X, PCP2.5X and PCP5X. The experimental rats in CSM(control), PCP1X, PCP2.5X and PCP5X were put through a timetable to cause CSM. PCP(20 μl) was administered at SP6, ST36 to groups PCP1X, PCP2.5X and PCP5X once every three days for a total of six times. The weight of the rats was measured by an electronic scale after PCP injection, once every three days for a total of six times. Blood samples were taken from the rats for analysis of aspartate aminotransferase(AST) and alanine aminotransferase(ALT). Serotonin and norepinephrine were measured by enzyme-linked immunosorbent assay(ELISA) kit.

Results:

The experimental groups(PCP1X, 2.5X, 5X) showed significant body weight increase compared to the control group. Regarding AST and ALT changes in serum, the experimental groups(PCP2.5X, 5X) showed a significant decrease compared to the control group. For Serotonin changes in serum, the experimental group(PCP5X) showed a significant increase compared to the control group. For Norepinephrine changes in serum, the experimental groups(PCP2.5X) showed a significant decrease compared to the control group.

Conclusions:

These data suggest that PCP at SP6, ST36 has therapeutic effects on CSM rats.

Fig. 1.

Effect of Poria cocos pharmacopuncture treatment on the body weight in CSM rats

CSM: chronic stress model.

##: p<0.01 compared with normal. **: p<0.01, *: p<0.05 compared with control.

Time Table for Chroinc Stress Induction in Rats

Changes on the Body Weight after Poria Cocos Pharmacopuncture Treatment in CSM Rats

Changes on the Aspartate Aminotransferase Contents after Poria Cocos Pharmacopuncture Treatment in CSM Rats

Changes on the Alanine Aminotransferase Contents after Poria Cocos Pharmacopuncture Treatment in CSM Rats

Changes on the Serotonin after Poria Cocos Pharmacopuncture Treatment in CSM Rats

Changes on the Norepinephrine after Poria Cocos Pharmacopuncture Treatment in CSM Rats

References

1. Seo SG. Adult disease · geriatrics Seoul: Korean Medicine Book Publisher; 1995. p. 6–7.
2. Burchfield S. The stress response, a new perspective. Psychosom 1979;Med 41:661–72.
3. Chrousos GP, Gold PW. The concept of stress and stress system disorders. Overview of physical and behavioral homeostasis. JAMA 1992;267(3):1244–52.
4. Stone EA. Stress and cathecholamines. In : Friedhoff AJ, ed. Cathecholamines and behavior New York: Plenum Press; 1975. p. 31–72.
5. Na CS. Korean medicine introduction Seoul: Euiseongdang Publishing Co; 2013. p. 463–4. p. 486.
6. The Korean Acupuncture & Moxibstion Medicine Society, The Textbook Compilation Committee. The Acupuncture and Moxibustion II Gyeonggi-do: Jipmoondang; 2008. p. 408.
7. The Korean Acupuncture & Moxibstion Medicine Society, The Textbook Compilation Committee. The Acupuncture and Moxibustion I Gyeonggi-do: Jipmoondang; 2008. p. 63–4. p. 80.
8. College of Oriental Medicine Herbal Medicine Compilation Committee. Herbal medicine Seoul: Younglimsa; 2008. p. 347–9.
9. Jeong JH, Choi CM, Seo YJ, Cho HB, Kim SB. The anti-depressive effect of Samul-tanggahyangbuja on chronic mild stress in ovariectomized rats. J Korean Obstet Gynecol 2013;26(4):30–47.
10. Kwon HJ, Kin KS, Kang SK, et al. Antistress effect of Gagamsanzointang on rat chronic stress model. Kor J Herbology 2012;27(6):29–35.
11. Kang MK, Kim YH, Im AR, Nam BS, Chae SW, Lee MY. Antidepressant-like effects of Nelumbo nucifera leaves extract in chronic mild stress model. Kor J Herbology 2014;29(2):7–13.
12. Moon BW, Lee HS. Effects of aqua-acupuncture of hoelen water extract on the renal function in rats. The Acupuncture 1997;14(2):199–207.
13. Kim MS, Kim BS, Yim YK. The effect of hoelen herbal-acupuncture at KI10 on lipopolysaccharide induced nephritis in rats. The Acupuncture 2014;31(2):65–74.
14. Willner P, Moreau JL, Nielsen CK, Papp M, Sluzewska A. Decreased hedonic responsiveness following chronic mild stress is not secondary to loss of body weight. Physiology & Behavior 1996;60(1):129–34.
15. Ko HJ. Stress management strategies in primary care. J Korean Acad Fam Med 2000;21(2):125–36.
16. Selye H. The stress of life Toronto: Longmans Green and Co; 1958. p. 1–50.
17. Cannon WB. The wisdom of the body Seoul: Dongmyoungsa; 2009. p. 1–98.
18. Iimori K. Changes in noradrenaline metabolism in rat brain regions by psychological stress. Japan Kurume Medical Society 1982;45(1):520.
19. Koob GF. Corticotropin-releasing factor, norepinephrine, and stress Amsterdam: Elsevier; 1999. p. 1167–80.
20. Lee KH. Clinical psychiatry Seoul: Hana Medicine Publishing Inc; 1988. p. 42. p. 148. p. 381. p. 501.
21. Min HK. Clinical endocrinology Seoul: Korean Medicine Book Publisher; 1999. p. 5. p. 31. p. 450.
22. Mason JW. A review of psychoendocrine research on the sympathetic-adrenal medulary system. Psychosomatic Medicine 1969;30:631–53.
23. Seo YH. Neurotransmitters Seoul: Minumsa; 1996. p. 115–92. p. 219–46.
24. Yang SH. Psychoneuroendocrinology of stress. Mental Health Research 1985;3(0):81–9.
25. Sunanda , Rao BS, Raju TR. Restraint stress induced alterations in the levels of biogenic amines, amino acids and AChE activity in the hippocampus. Neurochem RES 2000;25(120):1547–52.
26. Duman RS, Heninger GR, Nestler EJ. Amolecular and cellural theory of depression. Arch Gen Psychiatry 1997;54(7):597–606.
27. The Textbook Compilation Committee of Neuropsychiatry of Oriental Medical Schools in Nation. The neuropsychiatry of oriental medicine Seoul: Jipmoondang; 2005. p. 383–4.
28. Hong WS. Chinese history of medicine Seoul: Iljoongsa; 2001. p. 55.
29. Kim JW, Kim JH, Hwang EW. Comprehension of oriental medicine about stress. Journal of Oriental Paychiatry 1993;4(1):19–26.
30. Willner P. The valdity of animal model of depression. Psychopharmacology 1984;83(1):1–16.
31. Willner P. Valdity, reliability and utility of the chronic mild stress model of depression, a 10 year review and evauation. Psychopharmacology 1997;134(4):319–29.
32. Shin MK. Clinical herbal medicine Seoul: Younglimsa; 2002. p. 649–50.
33. Pharmacognosy Material Compilation Committee. Pharmacognosy Gyeonggi-do: Dongmyungsa; 2014. p. 708–10.
34. The Encyclopedia of Oriental Herbal Medicine Compilation Committee. The encyclopedia of oriental herbal medicine Seoul: Jungdam Publishing Inc; 2006. p. 1787–90.
35. Ahn YK. Meridianology series Seoul: Seongbosa; 2002. p. 196–7. p. 228–9.
36. Korean Acupuncture & Moxibustion Society Textbook Compilation Committee. The Acupuncture and Moxibustion III Gyeonggi-do: Jipmoondang; 2008. p. 391–417.
37. Kim SH, Lee WS, Lee HJ, et al. Effects of higher dietary cation with or without protected fat and niacin on the milk yield and thermoregulatory ability in holstein during heat stress. J Anim Sci & Technol 2006;48(5):555–62.
38. The Korean Society Pathologists. Pathology Seoul: Komoonsa; 2006. p. 1113.

Article information Continued

Fig. 1.

Effect of Poria cocos pharmacopuncture treatment on the body weight in CSM rats

CSM: chronic stress model.

##: p<0.01 compared with normal. **: p<0.01, *: p<0.05 compared with control.

Table 1.

Time Table for Chroinc Stress Induction in Rats

Mon Tue Wed Thu Fri
8: 30∼11: 00 LOF & WD TH & Tilt LOF & WD LOF & WD SS
11: 00∼12: 00 NB
12: 00∼13: 00
13: 00∼14: 00 NB NB FS Shaking FS
14: 00∼15: 00
15: 00∼16: 00 FS Shaking SS TH & Tilt TH & Tilt
16: 00∼17: 00
17: 00 0|후 TH TH TH TH TH

LOF: lights on at night. WD: water deprivation. NB: noise bursts. TH: tight husbandary, Tilt, Shaking, FS: forced swimming. SS: stroboscopic light.

Table 2.

Changes on the Body Weight after Poria Cocos Pharmacopuncture Treatment in CSM Rats

Treated times
Groups
CSM Poria cocos pharmacopuncture treatments
Before After 1 2 3 4 5 6
Nor 0.0±0.0 24.7±1.0 35.2 ±1.0 50.0 ±1.2 55.5 ±1.3 71.3±0.9 84.0±1.5 92.8±1.7
Con 0.0±0.0 23.0±1.6 21.5±2.6## 19.4±1.9## 34.9±2.3## 49.8±3.0## 62.8±3.8## 69.8±4.2##
PCP1X 0.0±0.0 17.7±0.8 23.0 ±1.6 33.0±1.4** 32.5±1.5 50.8±1.3 67.2±2.0 71.3±2.3
PCP2.5X 0.0±0.0 18.3±1.4 26.2 ±1.9 37.2±2.4** 41.8±2.3* 53.5±3.2 69.7±3.5 76.3 ±4.0
PCP5X 0.0±0.0 20.2±0.8 26.5 ±1.5 38.5±1.5** 43.2±2.3* 57.0±2.2 72.2±2.3* 79.7 ±3.0

Values are expressed mean±SE. CSM: chronic stress model.

##:

p<0.01 compared with normal.

**:

p<0.01,

*:

p<0.05 compared with control.

Table 3.

Changes on the Aspartate Aminotransferase Contents after Poria Cocos Pharmacopuncture Treatment in CSM Rats

Group Aspartate aminotransferase(U/l)
Nor 76.3±1.7
Con 96.6±7.0
PCP1X 77.5±4.4
PCP2.5X 73.8±1.7
PCP5X 75.0±0.9

Values are expressed mean±SE.

CSM: chronic stress model.

*:

p<0.05 compared with control.

Table 4.

Changes on the Alanine Aminotransferase Contents after Poria Cocos Pharmacopuncture Treatment in CSM Rats

Group Alanine aminotransterase(U/l)
Nor 25.5±1.1
Con 31.8±1.9
PCP1X 27.8±1.5
PCP2.5X 25.2±1.4*
PCP5X 22.8±0.8*

Values are expressed mean±SE.

CSM: chronic stress model.

*:

p<0.05 compared with control.

Table 5.

Changes on the Serotonin after Poria Cocos Pharmacopuncture Treatment in CSM Rats

Group Serotonin(ng/ml)
Nor 47.9±5.5
Con 29.0±2.1#
PCP1X 39.2±7.8
PCP2.5X 46.7±10.2
PC5X 53.2±8.9*

Values are expressed mean±SE.

CSM: chronic stress model.

#:

p<0.05 compared with normal.

*:

p<0.05 compared with control.

Table 6.

Changes on the Norepinephrine after Poria Cocos Pharmacopuncture Treatment in CSM Rats

Group Norepinephrine(pg/dl)
Nor 2.86±0.03
Con 2.90±0.04
PCP1X 2.49±0.20
PCP2.5X 2.56±0.08*
PC5X 2.53±0.08

Values are expressed mean±SE.

CSM: chronic stress model.

*:

p<0.05 compared with control.