Effects of Curculiginis Rhizoma Pharmaco-Acupuncture at ST36 on Monosodium Iodoacetate(MIA)-induced Osteoarthritic Rats

Article information

Acupunct. 2015;32(1):53-66
Department of Acupuncture & Moxibustion, Meridian & Acupoint, College of Oriental Medicine, Deagu Haany University
*Corresponding author: Department of Acupuncture & Moxibustion Medicine, Pohang Oriental Hospital of Daegu Haany University, 411, Saecheonnyeon-daero, Nam-gu, Pohang-si, Gyeongsangbuk-do, 790-826, Republic of Korea, Tel: +82-54-271-8010, E-mail: hanyeesarang@hanmail.net
Received 2015 February 09; Revised 2015 February 22; Accepted 2015 February 23.

Abstract

Objectives:

This study was designed to investigate effects of Curculiginis Rhizoma pharmaco- acupuncture at ST36 on monosodium iodoacetate-induced osteoarthritic rats.

Methods:

Twenty rats were divided into four groups consisting of 5 rats: rats receiving no injection(normal), rats injected with monosodium Iodoacetate(MIA, control), rats injected with MIA and normal saline(N-S), and rats injected with MIA and Curculiginis Rhizoma (CRPA). N-S and CRPA were administered once a day at ST36 during 21 days. After that we examined the weight-bearing ability of hind paws, liver and kidney function, immunocell, cytokines, proteins, and gene expression of cytokines. Injury of synovial tissue was measured by H & E, Safranin O immunofluorescence.

Results:

The weight-bearing ability of the hind paws, Serum TNF-α, IL-1β, IL-6, PGE2, LTB4, DPD, Osteocalcin, Protein COX-2 of CRPA decreased significantly. Protein Arachidonate 5 li- poxygenase of CRPA was decreased, but not significantly. Expression of gene COX-2, TNF-α, IL-1β, IL-6, NOS2 of CRPA decreased. In histological observations, CRPA was improved, compared with other control groups.

Conclusions:

It can be suggested that Curculiginis Rhizoma pharmaco-acupuncture at ST36 has anti-inflammatory and pain relief effects on monosodium iodoacetate-induced osteoarthritic rats.

Fig. 1.

Effects of CRPA on changes of weight bearing in hind paws of MIA-induced osteoarthritis rats

The results were expressed as mean ± SD(n=5).

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

*: statistically significant compared with control group (p<0.05).

Fig. 2.

Effects of CRPA on AST, ALT and Creatinine of MIA-induced osteoarthritis rats

The results were expressed as mean ± SD(n=5).

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

**: statistically significant compared with control group (p<0.01).

***: statistically significant compared with control group (p<0.001).

Fig. 3.

Effects of CRPA on WBC, neutronphi and monocyte in the blood of MIA-induced osteoarthritis rats

WBC: white blood cell.

The results were expressed as mean ± SD(n=5).

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

*: statistically significant compared with control group (p<0.05).

**: statistically significant compared with control group (p<0.01).

Fig. 4.

Effects of CRPA on inflammatory cytokines in the serum of MIA-induced osteoarthritis rats

The results were expressed as mean ± SD(n=5).

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

*: statistically significant compared with control group(p<0.05).

**: statistically significant compared with control group(p<0.01).

***: statistically significant compared with control group(p<0.001).

Fig. 5.

Effects of CRPA on COX-2 and Arachidonate 5 lipoxygenase content in synovial tissue of MIA-induced osteoarthritis rats

The results were expressed as mean ± SD(n=5).

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

*: statistically significant compared with control group(p<0.05).

Fig. 6.

Effects of CRPA on expression of inflammatory cytokine contents in synovial tissue of MIA-induced osteoarthritis rats

The results were expressed as mean ± SD(n=2).

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

Fig. 7.

Effects of CRPA on joint pathology(H & E) in joint tissue of MIA-induced osteoarthritis rats

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

Fig. 8.

Effets of CRPA on joint pathology (safranin O staining) in joint tissue of MIA- induced osteoarthritis rats

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

Effects of CRPA on Body Weight of MIA-induced Osteoarthritis Rats

Effects of CRPA on Changes of Weight Bearing in Hind Paws of MIA-induced Osteoarthritis Rats

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Article information Continued

Fig. 1.

Effects of CRPA on changes of weight bearing in hind paws of MIA-induced osteoarthritis rats

The results were expressed as mean ± SD(n=5).

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

*: statistically significant compared with control group (p<0.05).

Fig. 2.

Effects of CRPA on AST, ALT and Creatinine of MIA-induced osteoarthritis rats

The results were expressed as mean ± SD(n=5).

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

**: statistically significant compared with control group (p<0.01).

***: statistically significant compared with control group (p<0.001).

Fig. 3.

Effects of CRPA on WBC, neutronphi and monocyte in the blood of MIA-induced osteoarthritis rats

WBC: white blood cell.

The results were expressed as mean ± SD(n=5).

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

*: statistically significant compared with control group (p<0.05).

**: statistically significant compared with control group (p<0.01).

Fig. 4.

Effects of CRPA on inflammatory cytokines in the serum of MIA-induced osteoarthritis rats

The results were expressed as mean ± SD(n=5).

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

*: statistically significant compared with control group(p<0.05).

**: statistically significant compared with control group(p<0.01).

***: statistically significant compared with control group(p<0.001).

Fig. 5.

Effects of CRPA on COX-2 and Arachidonate 5 lipoxygenase content in synovial tissue of MIA-induced osteoarthritis rats

The results were expressed as mean ± SD(n=5).

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

*: statistically significant compared with control group(p<0.05).

Fig. 6.

Effects of CRPA on expression of inflammatory cytokine contents in synovial tissue of MIA-induced osteoarthritis rats

The results were expressed as mean ± SD(n=2).

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

Fig. 7.

Effects of CRPA on joint pathology(H & E) in joint tissue of MIA-induced osteoarthritis rats

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

Fig. 8.

Effets of CRPA on joint pathology (safranin O staining) in joint tissue of MIA- induced osteoarthritis rats

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

Table 1.

Effects of CRPA on Body Weight of MIA-induced Osteoarthritis Rats

Group Body weights(g)
0 7 14 21 days
Normal 297.8±4.31 333.7±6.94 363.5±6.72 371.5±4.12
Control 290.8±2.59 317.8±4.91 345.8±6.27 360.0±3.46
N-S 300.0±4.22 313.7±4.82 336.0±4.95 363.6±6.27
CRPA 309.6±5.80 312.8±7.99 341.2±9.97 365.8±10.34

The results were expressed as mean ± SD(n=5).

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

Table 2.

Effects of CRPA on Changes of Weight Bearing in Hind Paws of MIA-induced Osteoarthritis Rats

Group ratio
0 7 14 21 days
Normal 1.16±0.11 1.04±0.05 1.05±0.03 1.04±0.11
Control 0.61±0.10 4.06±1.23 2.48±0.20 2.60±0.47
N-S 0.86±0.14 2.37±0.36 1.78±0.25 1.80±0.12
CRPA 0.67±0.13 1.73±0.36 1.57±0.25* 1.12±0.15*

The results were expressed as mean ± SD(n=5).

Normal: rats were no injected with anyone.

Control: rats were injected with MIA.

N-S: rats were injected with MIA and normal saline.

CRPA: rats were injected with MIA and Curculiginis Rhizoma.

*

statistically significant compared with control group (p<0.05).