The Effect of Hoelen Herbal-acupuncture at KI10 on Lipopolysaccharide Induced Nephritis in Rats

Article information

Acupunct. 2014;31(2):65-74
Publication date (electronic) : 2014 June 20
doi : https://doi.org/10.13045/acupunct.2014025
Department of Meridian and Acupoint, College of Korean Medicine, Daejeon University
*Corresponding author : Department of Meridian and Acupoint, College of Korean Medicine, Daejeon University, 62, Daehak-ro, Dong-gu, Daejeon, 300-716, Republic of Korea Tel : +82-42-280-2610 E-mail : docwindy@dju.kr
Received 2014 May 13; Revised 2014 May 28; Accepted 2014 June 03.

Abstract

Objectives:

The purpose of this study is evaluating the effect of Hoelen Herbal-acupuncture (HO-HA) at KI10(Umgok) on Lipopolysaccharide(LPS) induced nephritis in rats

Methods:

The experimental rats were assigned to four groups; normal, LPS, saline, HO-HA groups. LPS(2 mg/kg) was administered to the rats in LPS, saline and HO-HA groups to induce acute inflammatory kidney damage. Saline injection and HO-HA were administered at KI10 three times a week.

Blood samples were taken from the rats for analysis of white blood cell(WBC), neutrophil, blood urea nitrogen(BUN), creatinine TNF-α, CINC-1. Urine samples were taken from the rats for analysis of urinal volume, creatinine and total protein. The kidney samples were taken from the rats for analysis of renal myeloperoxIdase(MPO).

Results:

HO-HA suppressed the increases of WBC and neutrophils in blood, BUN, creatinine, TNF-α and CINC-1 in serum, and MPO in kidney of LPS-stimulated rats. In addition, HO-HA inhibited the decrease of urinary volume in LPS-stimulated rats.

Conclusions:

HO-HA has therapeutic effects on LPS-induced inflammatory kidney damage in rats. Further studies may be needed for clinical use of HO-HA.

Fig. 1.

Effect of HO-HA on WBC count in blood of LPS-stimulated rats

Normal : normal SD rat.

LPS : LPS(2 mg/kg) challenge.

Saline : LPS(2 mg/kg) challenge and saline(200 μl/rat) injcetion at KI10.

HO-HA : LPS(2 mg/kg) challenge and HO-HA(2.5 %, 200 μl/rat) at KI10.

★★★ : p<0.001 compared to normal group by Kruskal-Wallis test.

††† : p<0.001, †: p<0.05 compared to LPS group by Kruskal-Wallis test.

Fig. 2.

Effect of HO-HA on neutrophil count in blood of LPS-stimulated rats

★★★ : p<0.001 compared to normal group by Kruskal-Wallis test.

††† : p<0.001 compared to LPS group by Kruskal-Wallis test.

###: p<0.001 compared to saline group by Kruskal-Wallis test.

Fig. 3.

Effects of HO-HA on serum BUN level in LPS-stimulated rats

★★★ : p<0.001 compared to normal group by Kruskal-Wallis test.

††† : p<0.001 compared to LPS group by Kruskal-Wallis test.

Fig. 4.

Effects of HO-HA on serum creatinine level in LPS-stimulated rats

★★★ : p<0.001 compared to normal group by Kruskal-Wallis test.

††† : p<0.001, † : p<0.05 compared to LPS group by Kruskal-Wallis test.

###: p<0.001 compared to saline group by Kruskal-Wallis test.

Fig. 5.

Effects of HO-HA on serum TNF-α level in LPS-stimulated rats.

★★★ : p<0.001 ★ : p<0.05 compared to normal group by Kruskal-Wallis test.

: p<0.05 compared to LPS group by Kruskal-Wallis test.

Fig. 6.

Effects of HO-HA on serum CINC-1 level in LPS-stimulated rats

★★ : p<0.01 compared to normal group by Kruskal-Wallis test.

: p<0.05 compared to LPS group by Kruskal-Wallis test.

Fig. 7.

Effect of HO-HA on urinary volume in LPS-stimulated rats

: p<0.05 compared to normal group by Kruskal-Wallis test.

: p<0.05 compared to LPS group by Kruskal-Wallis test.

## : p<0.01 compared to Saline group by Kruskal-Wallis test.

Fig. 8.

Effects of HO-HA on renal MPO level in LPS-stimulated rats

★★★ : p<0.001 compared to normal group by Kruskal- Wallis test.

Scheme 1.

Manufacturing procedure of Hoelen herbal acupuncture solution

Scheme 2.

Experimental procedure

Scheme 3.

Acquisition of experimental samples

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Article information Continued

Fig. 1.

Effect of HO-HA on WBC count in blood of LPS-stimulated rats

Normal : normal SD rat.

LPS : LPS(2 mg/kg) challenge.

Saline : LPS(2 mg/kg) challenge and saline(200 μl/rat) injcetion at KI10.

HO-HA : LPS(2 mg/kg) challenge and HO-HA(2.5 %, 200 μl/rat) at KI10.

★★★ : p<0.001 compared to normal group by Kruskal-Wallis test.

††† : p<0.001, †: p<0.05 compared to LPS group by Kruskal-Wallis test.

Fig. 2.

Effect of HO-HA on neutrophil count in blood of LPS-stimulated rats

★★★ : p<0.001 compared to normal group by Kruskal-Wallis test.

††† : p<0.001 compared to LPS group by Kruskal-Wallis test.

###: p<0.001 compared to saline group by Kruskal-Wallis test.

Fig. 3.

Effects of HO-HA on serum BUN level in LPS-stimulated rats

★★★ : p<0.001 compared to normal group by Kruskal-Wallis test.

††† : p<0.001 compared to LPS group by Kruskal-Wallis test.

Fig. 4.

Effects of HO-HA on serum creatinine level in LPS-stimulated rats

★★★ : p<0.001 compared to normal group by Kruskal-Wallis test.

††† : p<0.001, † : p<0.05 compared to LPS group by Kruskal-Wallis test.

###: p<0.001 compared to saline group by Kruskal-Wallis test.

Fig. 5.

Effects of HO-HA on serum TNF-α level in LPS-stimulated rats.

★★★ : p<0.001 ★ : p<0.05 compared to normal group by Kruskal-Wallis test.

: p<0.05 compared to LPS group by Kruskal-Wallis test.

Fig. 6.

Effects of HO-HA on serum CINC-1 level in LPS-stimulated rats

★★ : p<0.01 compared to normal group by Kruskal-Wallis test.

: p<0.05 compared to LPS group by Kruskal-Wallis test.

Fig. 7.

Effect of HO-HA on urinary volume in LPS-stimulated rats

: p<0.05 compared to normal group by Kruskal-Wallis test.

: p<0.05 compared to LPS group by Kruskal-Wallis test.

## : p<0.01 compared to Saline group by Kruskal-Wallis test.

Fig. 8.

Effects of HO-HA on renal MPO level in LPS-stimulated rats

★★★ : p<0.001 compared to normal group by Kruskal- Wallis test.

Scheme 1.

Manufacturing procedure of Hoelen herbal acupuncture solution

Scheme 2.

Experimental procedure

Scheme 3.

Acquisition of experimental samples