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J Acupunct Res > Volume 31(2); 2014 > Article
Kim, Kim, and Yim: The Effect of Hoelen Herbal-acupuncture at KI10 on Lipopolysaccharide Induced Nephritis in Rats
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Abstract

Objectives:

The purpose of this study is evaluating the effect of Hoelen Herbal-acupuncture (HO-HA) at KI10(Umgok) on Lipopolysaccharide(LPS) induced nephritis in rats

Methods:

The experimental rats were assigned to four groups; normal, LPS, saline, HO-HA groups. LPS(2 mg/kg) was administered to the rats in LPS, saline and HO-HA groups to induce acute inflammatory kidney damage. Saline injection and HO-HA were administered at KI10 three times a week.
Blood samples were taken from the rats for analysis of white blood cell(WBC), neutrophil, blood urea nitrogen(BUN), creatinine TNF-α, CINC-1. Urine samples were taken from the rats for analysis of urinal volume, creatinine and total protein. The kidney samples were taken from the rats for analysis of renal myeloperoxIdase(MPO).

Results:

HO-HA suppressed the increases of WBC and neutrophils in blood, BUN, creatinine, TNF-α and CINC-1 in serum, and MPO in kidney of LPS-stimulated rats. In addition, HO-HA inhibited the decrease of urinary volume in LPS-stimulated rats.

Conclusions:

HO-HA has therapeutic effects on LPS-induced inflammatory kidney damage in rats. Further studies may be needed for clinical use of HO-HA.

Fig. 1.
Effect of HO-HA on WBC count in blood of LPS-stimulated rats
Normal : normal SD rat.
LPS : LPS(2 mg/kg) challenge.
Saline : LPS(2 mg/kg) challenge and saline(200 μl/rat) injcetion at KI10.
HO-HA : LPS(2 mg/kg) challenge and HO-HA(2.5 %, 200 μl/rat) at KI10.
★★★ : p<0.001 compared to normal group by Kruskal-Wallis test.
††† : p<0.001, †: p<0.05 compared to LPS group by Kruskal-Wallis test.
acupunct-31-2-65-7f1.gif
Fig. 2.
Effect of HO-HA on neutrophil count in blood of LPS-stimulated rats
★★★ : p<0.001 compared to normal group by Kruskal-Wallis test.
††† : p<0.001 compared to LPS group by Kruskal-Wallis test.
###: p<0.001 compared to saline group by Kruskal-Wallis test.
acupunct-31-2-65-7f2.gif
Fig. 3.
Effects of HO-HA on serum BUN level in LPS-stimulated rats
★★★ : p<0.001 compared to normal group by Kruskal-Wallis test.
††† : p<0.001 compared to LPS group by Kruskal-Wallis test.
acupunct-31-2-65-7f3.gif
Fig. 4.
Effects of HO-HA on serum creatinine level in LPS-stimulated rats
★★★ : p<0.001 compared to normal group by Kruskal-Wallis test.
††† : p<0.001, † : p<0.05 compared to LPS group by Kruskal-Wallis test.
###: p<0.001 compared to saline group by Kruskal-Wallis test.
acupunct-31-2-65-7f4.gif
Fig. 5.
Effects of HO-HA on serum TNF-α level in LPS-stimulated rats.
★★★ : p<0.001 ★ : p<0.05 compared to normal group by Kruskal-Wallis test.
: p<0.05 compared to LPS group by Kruskal-Wallis test.
acupunct-31-2-65-7f5.gif
Fig. 6.
Effects of HO-HA on serum CINC-1 level in LPS-stimulated rats
★★ : p<0.01 compared to normal group by Kruskal-Wallis test.
: p<0.05 compared to LPS group by Kruskal-Wallis test.
acupunct-31-2-65-7f6.gif
Fig. 7.
Effect of HO-HA on urinary volume in LPS-stimulated rats
: p<0.05 compared to normal group by Kruskal-Wallis test.
: p<0.05 compared to LPS group by Kruskal-Wallis test.
## : p<0.01 compared to Saline group by Kruskal-Wallis test.
acupunct-31-2-65-7f7.gif
Fig. 8.
Effects of HO-HA on renal MPO level in LPS-stimulated rats
★★★ : p<0.001 compared to normal group by Kruskal- Wallis test.
acupunct-31-2-65-7f8.gif
Scheme 1.
Manufacturing procedure of Hoelen herbal acupuncture solution
acupunct-31-2-65-7f9.gif
Scheme 2.
Experimental procedure
acupunct-31-2-65-7f10.gif
Scheme 3.
Acquisition of experimental samples
acupunct-31-2-65-7f11.gif

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