Comparison Study for Effects of Pharmacopunctures of GB40 on Weight Bearing Ratio in Grade III Ankle Sprain Model

Article information

Acupunct. 2014;31(2):11-19
Publication date (electronic) : 2014 June 20
doi : https://doi.org/10.13045/acupunct.2014020
1Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Wonkwang University
2Department of Physiology, College of Korean Medicine, Wonkwang University
3Department of Meridian & Acupoint, College of Korean Medicine, Wonkwang University
* Corresponding author : Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Wonkwang University, 895, Muwang-ro, Iksan-si, Jeollabuk-do, 570-754, Republic of Korea, Tel : +82-63-859-2812 E-mail : choandle@hanmail.net
Received 2014 April 24; Revised 2014 April 24; Accepted 2014 May 26.

Abstract

Objectives:

This study was aimed to determine which pharmacopuncture was effective in relieving pain in standard pain model, acute grade 3 ankle sprain rat model.

Methods:

Percentage changes of weight bearing ratio(WBR), as pain indicator, were measured after anti-inflammation, neutro Eohyul, Hwangryunhaedock-decoction pharmacopuncture were injected to Guheo(GB40).

Results:

In three kinds of pharmacopuncture, neutro Eohyul and Hwangryunhaedock-decoction pharmacopuncture represented more effect in percentage changes of WBR than surgical(non treatment), needling group.

Conclusions:

Comparing of pharmacopuncture in grade 3 ankle sprain rat model, these results provide basis to select pharmacopuncture in sprain disease. Furthermore, mechanism of pharmacopuncture’s analgesia, anti-inflammation should be progressed.

Fig. 1.

Comparison of normal group and ankle surgical sprain group

Weight bearing ratio stands for foot steping force to body weight.

Foot steping force were measured as highest foot stepping force of the hind limb of ankle sprained rat.

The data were expressed mean ± SE of weingt bearing ratio.

Fig. 2.

Effect of anti-inflammation pharmaco-puncture applied to GB40 on changes of WBR ratio in a ankle sprain rat

Control group were consisted of sprain group(non treated group) and needling group.

Needling group were needled to GB40 in 5 seconds. Anti-inflammation group were injected anti-inflammation pharmacopuncture 50 μl to GB40 in 5 seconds.

The data were expressed mean ± SE of percent changes in weight bearing ratio.

* : statistically significant compared with sprain group(*:p<0.05)

Fig. 3.

Effect of neutro Eohyul pharmaco-puncture applied to GB40 on changes of WBR ratio in a ankle sprain rat

Neutro Eohyul group were injected neutro Eohyul pharmaco-puncture 50 μl to GB40 in 5 seconds.

The data were expressed mean ± SE of percent changes in weight bearing ratio.

*, # : statistically significant compared with sprain group, needling group respectively(*, # : p<0.05)

Fig. 4.

Effect of Hwangryunhaedock-decoction pharmacopuncture applied to GB40 on changes of WBR ratio in a ankle sprain rat

Hwangryunhaedock-decoction group were injected Hwangryunhaedock-decoction pharmacopuncture 50 μl to GB40 in 5 seconds.

The data were expressed mean ± SE of percent changes in weight bearing ratio.

*, # : statistically significant compared with sprain group, needling group respectively(*, # : p<0.05)

Fig. 5.

Comparison of neutro Ehoyul and Hwang-ryunhaedock-decoction pharmacopuncture applied to GB40 on changes of WBR ratio in a ankle sprain rat

The data were expressed mean ± SE of percent changes in weight bearing ratio.

Notes

This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education(NRF-2012R1A1A4A01004637)

References

1. You TS, Park DS, Kang SK. Clinical observations on treatment of ankle-sprain. J Korean Oriental Med 2004;21(1):169.
2. Brozman SB, Wilk KE. Clinical orthopaedic rehabilitation : an evidence-based approach 3rd edth ed. Seoul: Hanmi Medicine; 2012. p. 332.
3. Kang JH, Heo DS, Yoon IJ, Oh MS. An Analysis of tendencies of studies on herbal acupuncture - focusing on domestic theses since 2001 about anti-inflammation, pain relief and anti-obesity effects, including safety-. J Korean Oriental Med 2007;28(2):93–113.
4. Yoon MY, Yoo HS. Inhibitory effects of Soyeum pharmacopuncture(SPP) on LPS-induced inflammation related cytokine expressions of RAW 264.7 cells. Journal of Pharmacopuncture 2007;10(3):21–28.
5. Lee SG, Won JK, Yeom SR, Lee SK, Song YS, Kwon YD. The effects of Dokhwalgisaeng- tang(Duhuoqisheng-tang) and Jungsongouhyul pharmacopuncture on pain control and nerve regeneration in the crush-induced sciatic nerve injury of the rat model. J Oriental Rehab Med 2009;19(3):15–32.
6. Shin GC, Cho KH, Kim YS, Bai HS, Lee KS. Studies on efficacy and safety of drug-acupuncture with Hwangryunhaedoktang. Kyung Hee University Oriental Medicine Journal 1994;17(1):85–119.
7. Koo ST, Park YI, Lim KS, Chung K, Chung JM. Acupuncture analgesia in a new rat model of ankle sprain pain. Pain 2002;99(3):423–31.
8. Kim I, An SH, Koo ST, Kim SY, Kim KS, Shon IC. Effect of Acupuncture applied to Hand Yang meridian on the rat model of ankle sprain pain. The Korean Journal of Meridian & Acupoint 2004;21(4):69–82.
9. Kim SY, Koo ST, Kim KS, Shon IC. Effect of electro-acupuncture applied to the Won point and Rak point in the rat model of ankle sprain pain. The Korean Journal of Meridian & Acupoint 2005;22(1):7–21.
10. Park SY, Choi YY, Jeon IS, et al. Capsaicin pharm-acopuncture modulates ankle sprain induced pain in rats. The Korean Journal of Meridian & Acupoint 2006;23(2):113–23.
11. Koo ST, Cho MS, Park SS, et al. Effect of frutus gardeniae herbal acupuncture on the rat model of ankle sprain pain. The Korean Journal of Meridian & Acupoint 2005;22(2):57–74.
12. Kim HY, Wang J, Chung K, Chung JM. A surgical ankle sprain pain model in the rat: effect of morphine and incomethacin. Neuroscience Letters 2008;442(2):161–4.
13. Colleges of Korean Medicine. Practice of acupuncture. Jongryeo 2010;:512.
14. Kim JH, Kim HY, Chung K, Chung JM. Electro-acupuncture reduces the evoked responses of the spinal dorsal horn neurons in ankle-sprained rats. J Neurophysiol 2011;105(5):2050–7.
15. Baker HB, Beynnon BD, Renstron PA. Ankle injury risk factors in sports. Sports Medicine 1997;23(2):69–74.
16. Wolfe Michael W, Uhl TIM L, Mattacola Carl G, Mccluskey Leland C. Management of ankle sprains 2001;63(1):93–105.
17. Cho NG, Kim KS. Studies of acupuncture in sprain disease. The Journal of Korean Acupuncture & Moxibustion Medicine Society 1995;12(2):73–4.
18. Korean Pharmacopuncture institute. Pharmaco-puncturology Seoul: ElsevierKorea; 2011. 1p. 163–80. p. 222–4.
19. Baik SI, Ahn JC, Kim YJ, Kim HS, Kwon KR. Type Analysis of Pharmacopuncture Papers Published in the Journal of Korean Acupuncture and Moxibustion. The Journal of Korean Acupuncture & Moxibustion Medicine Society 2006;23(6):19–27.
20. Korean Acupuncture & Moxibustion Medicine Society. The acupuncture and moxibustion medicine Gyeonggi: Jipmoondang; 2012. p. 573.
21. Lee IG, Yoo SH, Choi HS, Ryu SH. The clinical observations of 62 cases of ankle sprains treated with acupuncture. The Journal of Korean Acupuncture & Moxibustion Society 1990;7(1):177–85.
22. Xinnong Cheng. Chinese acupuncture and moxi-bustion. Yinyangmacjin Seoul 1997;:686–7.
23. Jang MK, Yoon EH, Jung CY, et al. Review of randomized controlled trials on pharmaco-puneture treatment for musculoskeletal diseases. The Journal of Korean Acupuncture & Moxi-bustion Society 2009;26(3):149–63.
24. Shin MK. Clinical traditional herbalogy. Young-lim 2006;:622–3.
25. Lee HJ. Pharmacopuncture gaeron & Imsang (introduction & clinical) Seoul: Iljoong; 1999. p. 13–15.
26. Kim SL, Hong KW. The clinical observations of 3 cases of metatarsal tendinitis treated with anti- inflammatory herbal acupuncture. Journal of Pharmacopuncture 2007;10(1):157–62.
27. Choi YJ, Kim JH, Yoon KJ, et al. Comparative study of BUM pharmacopuncture and Soyeom pharmacopunture on peripheral facial paralysis with postauricular pain. The Journal of Korean Acupuncture & Moxibustion Medicine Society 2012;29(5):31–37.
28. Lm JG, Lee JB, Lee HG, Yook TH, Kim JU. Effects of the acupuncture therapy in combination with Soyeom pharmacopuncture therapy on acute whiplash injury by traffic accident. The Journal of Korean Acupuncture & Moxibustion Society 2011;28(4):9–18.
29. Jeong MJ, Kwon OG, Woo CH, An HD. Dose dependent effects of Jungsongouhyul pharm-acopuncture on low back pain. Journal of Pharmacopuncture 2011;14(2):45–52.
30. Yoon IJ. Effect of Jungsonguhyul herbal acupuncture therapy for neck pain patients. The Journal of Daejeon Oriental Medicine 2007;16(2):1–5.
31. Cho JY, Kim YJ, Kim EJ, Lee SD, Kim KS. The effects of pharmacopuncture by the anti-inflammatory action of suppression of iNOS production on mice with allergic rhinitis. The Journal of Korean Acupuncture & Moxibustion Society 2012;29(1):89–101.
32. Hong CH. Two cases of seborrheic dermatitis treated by Hwangryunhaedok-tang pharmaco-puncture therapy. The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology 2012;25(2):68–75.
33. Jang HH, Lee DY, Lee SH, Kang HW, Lyu YS. Clinical studies on Hwangryunhaedoktang herbal acupuncture therapy on headache. Journal of Pharmacopuncture 2003;6(1):52–60.
34. Lee JS, Lee SJ, Nam JH, et al. The comparative study on the effect of pharmacopuncture treatment, Chuna treatment, pharmacopuncture - Chuna cooperative treatment for neck pain caused by traffic accidents. The Journal of Korean Acupuncture & Moxibustion Medicine Society 2012;29(5):87–95.

Article information Continued

Fig. 1.

Comparison of normal group and ankle surgical sprain group

Weight bearing ratio stands for foot steping force to body weight.

Foot steping force were measured as highest foot stepping force of the hind limb of ankle sprained rat.

The data were expressed mean ± SE of weingt bearing ratio.

Fig. 2.

Effect of anti-inflammation pharmaco-puncture applied to GB40 on changes of WBR ratio in a ankle sprain rat

Control group were consisted of sprain group(non treated group) and needling group.

Needling group were needled to GB40 in 5 seconds. Anti-inflammation group were injected anti-inflammation pharmacopuncture 50 μl to GB40 in 5 seconds.

The data were expressed mean ± SE of percent changes in weight bearing ratio.

* : statistically significant compared with sprain group(*:p<0.05)

Fig. 3.

Effect of neutro Eohyul pharmaco-puncture applied to GB40 on changes of WBR ratio in a ankle sprain rat

Neutro Eohyul group were injected neutro Eohyul pharmaco-puncture 50 μl to GB40 in 5 seconds.

The data were expressed mean ± SE of percent changes in weight bearing ratio.

*, # : statistically significant compared with sprain group, needling group respectively(*, # : p<0.05)

Fig. 4.

Effect of Hwangryunhaedock-decoction pharmacopuncture applied to GB40 on changes of WBR ratio in a ankle sprain rat

Hwangryunhaedock-decoction group were injected Hwangryunhaedock-decoction pharmacopuncture 50 μl to GB40 in 5 seconds.

The data were expressed mean ± SE of percent changes in weight bearing ratio.

*, # : statistically significant compared with sprain group, needling group respectively(*, # : p<0.05)

Fig. 5.

Comparison of neutro Ehoyul and Hwang-ryunhaedock-decoction pharmacopuncture applied to GB40 on changes of WBR ratio in a ankle sprain rat

The data were expressed mean ± SE of percent changes in weight bearing ratio.