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J Acupunct Res > Volume 31(1); 2014 > Article
Yeo, Kim, and Lee: Study on a Single Oral Dose Toxicity Test of Horse Placenta Hydrolysate Extract in Sprague-Dawley Rats
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Abstract

Objectives :

The objective of this study is to analyze the single-dose toxicity of horse placenta hydrolysate extracts.

Methods :

Sprague-Dawley rats were chosen for the study. Doses of horse placenta hydrolysate extracts, 2,000 mg/kg, 1,000 mg/kg and 500 mg/kg, were administered to the experimental group, and the same doses of normal saline solution were administered to the control group. This study was conducted under the approval of the Institutional Animal Ethics Committee.

Results :

In all 4 groups, no deaths occurred, and the horse placenta hydrolysate extracts administered by oral was over 2,000 mg/kg.
No significant changes in the weight between the control group and the experimental group were observed.
To check for abnormalities in organs and tissues, we used microscopy to examine representative histological sections of each specified organ, the results showed no significant differences in any organs or tissues.

Conclusions :

The above findings suggest that treatment with horse placenta hydrolysate extracts is relatively safe. Further studies on this subject should be conducted to yield more concrete evidence.

Table 1.
Mortality of the Rats Orally Treated with Horse Placenta Hydrolysate Extract Once
Sex Dose (mg/kg) No. of rats Hours after administration Days after administration Final mortality
1 2 3 4 5 6 3 6 10 11 12 13 14
Male Control 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0/5
500 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0/5
1,000 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0/5
2,000 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0/5
Table 2.
Clinical Signs of the Rats Orally Treated with Horse Placenta Hydrolysate Extract Once
Sex Dose (mg/kg) Clinical signs Days after administration
0 1 2 3 4 5 6 7
Male Control NAD 5 5 5 5 5 5 5 5
500 NAD 5 5 5 5 5 5 4 5
1,000 NAD 5 5 5 5 5 5 5 5
2,000 NAD 5 5 5 5 5 5 5 5

NAD : no abnormalities detected.

Table 3.
Body Weights of the Rats Orally Treated with Horse Placenta Hydrolysate Extract Once
Sex Dose (mg/kg) Number of rats Days after administration
0 7 14
Male Control 5 Mean 178.7 197.3 257.3
SD 5.82 7.18 6.33
500 5 Mean 178.2 191.25 251.32
SD 6.23 8.19 8.88
1000 5 Mean 176.8 195.65 259.32
SD 5.24 5.84 9.94
2000 5 Mean 179.5 180.26 254.32
SD 6.12 9.05 5.85

SD : standard deviation.

Table 4.
Gross Findings of the Rats Orally Treated with Horse Placenta Hydrolysate Extract Once
Incidence of gross findings
Male
Dose(mg/kg) Control 500 1,000 2,000
No. of rats 5 5 5 5
Items
Adrenal gland NGF 5(100 %) 5(100 %) 5(100 %) 5(100 %)
Brain NGF 5(100 %) 5(100 %) 5(100 %) 5(100 %)
Heart NGF 5(100 %) 5(100 %) 5(100 %) 5(100 %)
Liver NGF 5(100 %) 5(100 %) 5(100 %) 5(100 %)
Kidney NGF 5(100 %) 5(100 %) 5(100 %) 5(100 %)
Spleen NGF 5(100 %) 5(100 %) 5(100 %) 5(100 %)
Testis NGF 5(100 %) 5(100 %) 5(100 %) 5(100 %)
Thymus NGF 5(100 %) 5(100 %) 5(100 %) 5(100 %)

NGF : no gross finding. ( ) : percent of no gross finding.

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