Effects of Curcuma longa LINNE Pharmacopuncture at ST36 on Adjuvant Induced Arthritis in Rats

Article information

J Korean Acupunct Moxib Soc. 2013;30(4):95-105
Publication date (electronic) : 2013 September 20
doi : https://doi.org/10.13045/acupunct.2013026
1Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Dongshin University
2Department of Sasang Constitutional Medicine, College of Oriental Medicine, Dongshin University
*Corresponding author : Department of Acupuncture & Moxibustion Medicine, Mokpo Oriental Hospital of Dongshin University, 313, Baengnyeon-daero, Mokpo-si, Jeollanam-do, 530-822, Republic of Korea, Tel : +82-61-280-7903 E-mail : fire-sjc@hanmail.net
Received 2013 August 08; Revised 2013 August 25; Accepted 2013 August 26.

Abstract

Objectives :

The objective of this study was to investigate the effects of Curcuma longa LINNE pharmacopuncture at ST36 on Complete Freund’s Adjuvant(CFA)-induced arthritis in rats.

Materials and methods :

Arthritis was induced by injecting CFA subcutaneously into the left knee joint and paw, and Curcuma longa LINNE pharmacopuncture(CLL-A. 0.0343 μg/kg; CLL-B. 0.171 μg/kg; CLL-C. 0.343 μg/kg) was injected at ST36 each other day for 5 times beginning on day 10 after the CFA injection. Paw edema, withdrawal response, hematological, serological and histological observation were assessed.

Results :

In paw edema volume all 3 groups(CLL-A, CLL-B, CLL-C) showed significant decrease compared to the CFA control group. In withdrawal response to reaction time and withdrawal response to force all 3 groups(CLL-A, CLL-B, CLL-C) showed significant increase compared to the CFA control group. In serum AST, group CLL-C showed significant decrease compared to the CFA control group. In histological observations, in all 3 groups, more normal chondrocytes were observed compared to the CFA control group and safranin O stain showed high positive reaction in the cartilage tissue close to the bone tissue.

Conclusions :

The results suggest that Curcuma longa LINNE Pharmacopuncture at ST36 has a suppressing inflammation effect on Freund’s adjuvant arthritis in rats.

Fig. 1.

Changes on the paw edema volume after CLL phamacopuncture at Joksamri acupoint in CFA induced arthritis rats

A description of controls and acupuncture groups refer to Table 1.

Changes on the Paw Edema Volume after CLL Phamacopuncture at Joksamri Acupoint in CFA Induced Arthritis Rats

##p<0.01 compared with intact control.

*p<0.05, **p<0.01 compared with CFA control.

Fig. 2.

Changes on the withdrawal response to reaction time after CLL phamacopuncture at Joksamri acupoint in CFA induced arthritis rats

Values are expressed mean±SE.

A description of controls and acupuncture groups refer to Table 1.

* : p<0.05, ** : p<0.01 compared with CFA control.

Fig. 3.

Changes on the withdrawal response to force after CLL phamacopuncture at Joksamri acupoint in CFA induced arthritis rats

Values are expressed mean±SE. A description of controls and acupuncture groups refer to Table 1.

* : p<0.05, ** : p<0.01 compared with CFA control.

Fig. 4.

Changes on the histopathological change (HE stain) of knee joint after CLL phamacopuncture at Joksamri acupoint in CFA induced arthritis rats

A : intact control.

B : CFA control.

C : CLL-A, CLL 10 % phamacopuncture at Joksamri(ST36).

D : CLL-B, CLL 50 % phamacopuncture at Joksamri(ST36).

E : CLL-C, CLL 100 % phamacopuncture at Joksamri(ST36).

HE-stain, scale bars = 100 μm.

Fig. 5.

Changes on the histopathological change (safranin O-fast stain) of knee joint after CLL pham-acopuncture at Joksamri acupoint in CFA induced arthritis rats

A : intact control.

B : CFA control.

C : CLL-A, CLL 10 % phamacopuncture at Joksamri(ST36).

D : CLL-B, CLL 50 % phamacopuncture at Joksamri(ST36).

E : CLL-C, CLL 100 % phamacopuncture at Joksamri(ST36).

Safranin O-fast stain, scale bars = 100 μ.

Changes on the Withdrawal Response to Reaction Time after CLL Phamacopuncture at Joksamri Acupoint in CFA Induced Arthritis Rats (Sec)

Changes on the Withdrawal Response to Force after CLL Phamacopuncture at Joksamri Acupoint in CFA Induced Arthritis Rats (g)

Changes on the Aspartate Aminotransferase Contents after CLL Phamacopuncture at Joksamri Acupoint in CFA Induced Arthritis Rats

Changes on the Alanine Amino-transferase Contents after CLL Phamacopuncture at Joksamri Acupoint in CFA Induced Arthritis Rats

Changes on the Blood WBC Contents after CLL Phamacopuncture at Joksamri Acupoint in CFA Induced Arthritis Rats

Changes on the Blood RBC, HGB, HCT, MCV, MCH, MCHC and PLT Contents after CLL Phamacopuncture at Joksamri Acupoint in CFA Induced Arthritis Rats

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Article information Continued

Fig. 1.

Changes on the paw edema volume after CLL phamacopuncture at Joksamri acupoint in CFA induced arthritis rats

A description of controls and acupuncture groups refer to Table 1.

Changes on the Paw Edema Volume after CLL Phamacopuncture at Joksamri Acupoint in CFA Induced Arthritis Rats

##p<0.01 compared with intact control.

*p<0.05, **p<0.01 compared with CFA control.

Fig. 2.

Changes on the withdrawal response to reaction time after CLL phamacopuncture at Joksamri acupoint in CFA induced arthritis rats

Values are expressed mean±SE.

A description of controls and acupuncture groups refer to Table 1.

* : p<0.05, ** : p<0.01 compared with CFA control.

Fig. 3.

Changes on the withdrawal response to force after CLL phamacopuncture at Joksamri acupoint in CFA induced arthritis rats

Values are expressed mean±SE. A description of controls and acupuncture groups refer to Table 1.

* : p<0.05, ** : p<0.01 compared with CFA control.

Fig. 4.

Changes on the histopathological change (HE stain) of knee joint after CLL phamacopuncture at Joksamri acupoint in CFA induced arthritis rats

A : intact control.

B : CFA control.

C : CLL-A, CLL 10 % phamacopuncture at Joksamri(ST36).

D : CLL-B, CLL 50 % phamacopuncture at Joksamri(ST36).

E : CLL-C, CLL 100 % phamacopuncture at Joksamri(ST36).

HE-stain, scale bars = 100 μm.

Fig. 5.

Changes on the histopathological change (safranin O-fast stain) of knee joint after CLL pham-acopuncture at Joksamri acupoint in CFA induced arthritis rats

A : intact control.

B : CFA control.

C : CLL-A, CLL 10 % phamacopuncture at Joksamri(ST36).

D : CLL-B, CLL 50 % phamacopuncture at Joksamri(ST36).

E : CLL-C, CLL 100 % phamacopuncture at Joksamri(ST36).

Safranin O-fast stain, scale bars = 100 μ.

Table 1.

Changes on the Paw Edema Volume after CLL Phamacopuncture at Joksamri Acupoint in CFA Induced Arthritis Rats

Days(times) 0 10 12 (1st) 14 (2nd) 16 (3rd) 18 (4th) 20 (5th)
Group
Controls Intact (n=5) 1.74±0.06 1.89±0.03 1.83±0.05 1.92±0.06 1.91±0.04 1.97±0.05 2.04±0.04
CFA (n=5) 1.90±0.05 3.43±0.23## 3.40±0.14## 3.39±0.15## 3.64±0.19## 3.56±0.19## 3.67±0.20##
Acupuncture CLL-A(n=5) 1.89±0.04 3.78±0.22 3.23±0.14 3.28±0.12 3.16±0.15 2.95±0.18* 2.83±0.12**
CLL-B (n=5) 1.89±0.03 3.97±0.27 3.46±0.16 3.31±0.14 3.16±0.10 2.90±0.14* 2.89±0.09*
CLL-C (n=5) 2.04±0.05 3.41±0.19 3.14±0.18 3.03±0.17 3.07±0.13* 2.69±0.07** 2.65±0.07**

Values are expressed mean±SE.

CFA : complete Freund’s adjuvant. CLL-A : CLL 10 % phamacopuncture at Joksamri(ST-36).

CLL-B : CLL 50 % phamacopuncture at Joksamri(ST-36). CLL-C : CLL 100 % phamacopuncture at Joksamri(ST-36).

##:

p<0.01 compared with intact control.

*:

p<0.05,

**:

p<0.01 compared with CFA control.

Table 2.

Changes on the Withdrawal Response to Reaction Time after CLL Phamacopuncture at Joksamri Acupoint in CFA Induced Arthritis Rats (Sec)

Days(times) 0 10 12 (1st) 14 (2nd) 16 (3rd) 18 (4th) 20 (5th)
Group
Controls CFA(n=5) 6.0±0.5 2.8±0.6 3.3±0.5 3.0±0.6 2.7±0.4 2.3±0.4 1.7±0.2
Acupuncture CLL-A (n=5) 5.6±0.6 2.5±0.5 3.2±0.4 4.2±0.5 3.2±0.3 3.6±0.3* 4.4±0.4**
CLL-B (n=5) 5.2±0.3 2.7±0.6 3.1±0.5 3.7±0.4 3.8±0.2* 3.7±0.2* 4.5±0.3**
CLL-C (n=5) 5.7±0.2 2.1±0.4 3.2±0.3 3.8±0.2 3.6±0.3 4.6±0.4** 4.2±0.6**

Values are expressed mean±SE. A description of controls and acupuncture groups refer to Table 1.

*:

p<0.05,

**:

p<0.01 compared with CFA control.

Table 3.

Changes on the Withdrawal Response to Force after CLL Phamacopuncture at Joksamri Acupoint in CFA Induced Arthritis Rats (g)

Days(times) 0 10 12 (1st) 14 (2nd) 16 (3rd) 18 (4th) 20 (5th)
Group
Controls CFA (n=5) 29.9±2.7 14.2±3.2 16.6±2.5 15.3±2.8 13.6±1.9 11.9±1.8 9.1±1.1
Acupuncture CLL-A (n=5) 28.4±2.8 13.0±2.5 16.5±1.8 21.2±2.4 16.3±1.3 18.4±1.6* 22.2±2.1**
CLL-B (n=5) 26.3±1.5 13.6±3.1 16.1±1.9 18.7±1.8 19.5±1.0* 19.0±1.2* 22.7±1.6**
CLL-C (n=5) 28.5±1.0 10.8±2.0 16.3±1.6 19.2±1.1 18.4±1.3 23.2±2.1** 24.2±1.7**

Values are expressed mean±SE.

A description of controls and acupuncture groups refer to Table 1.

*:

p<0.05,

**:

p<0.01 compared with CFA control.

Table 4.

Changes on the Aspartate Aminotransferase Contents after CLL Phamacopuncture at Joksamri Acupoint in CFA Induced Arthritis Rats

Group Aspartate aminotransferase (U/l)
Controls Intact (n=5) 81.6±15.0
CFA (n=5) 118.0±6.1##
Acupuncture CLL-A (n=5) 109.8±8.0
CLL-B (n=5) 115.4±6.1
CLL-C (n=5) 85.8±2.6**

Values are expressed mean±SE.

A description of controls and acupuncture groups refer to Table 1.

##:

p<0.01 compared with intact control.

**:

p<0.01 compared with CFA control.

Table 5.

Changes on the Alanine Amino-transferase Contents after CLL Phamacopuncture at Joksamri Acupoint in CFA Induced Arthritis Rats

Group Aspartate aminotransferase (U/l)
Controls Intact (n=5) 27.8±4.9
CFA (n=5) 32.3±2.5
Acupuncture CLL-A (n=5) 31.6±1.4
CLL-B (n=5) 33.8±2.6
CLL-C (n=5) 28.0±0.6

Values are expressed mean±SE.

A description of controls and acupuncture groups refer to Table 1.

Table 6.

Changes on the Blood WBC Contents after CLL Phamacopuncture at Joksamri Acupoint in CFA Induced Arthritis Rats

Group WBC(K/uL) Neutrophil(K/uL) Lymphocyte(K/uL) Monocyte(K/uL) Eosinphil(K/uL)
Controls Intact (n=5) 5.1±0.8 1.4±0.2 3.7±0.56 0.28±0.050 0.018±0.004
CFA (n=5) 7.28±0.87 2.16±0.18 4.55±0.74 0.53±0.14 0.04±0.01
Acupuncture CLL-A (n=5) 6.56±1.01 2.06±0.29 3.95±0.75 0.52±0.08 0.02±0.004
CLL-B (n=5) 5.65±0.72 1.90±0.20 3.38±0.77 0.36±0.06 0.02±0.01
CLL-C (n=5) 5.73±0.32 1.78±0.23 3.49±0.19 0.45±0.05 0.01±0.003

Values are expressed mean±SE.

A description of controls and acupuncture groups refer to Table 1.

Table 7.

Changes on the Blood RBC, HGB, HCT, MCV, MCH, MCHC and PLT Contents after CLL Phamacopuncture at Joksamri Acupoint in CFA Induced Arthritis Rats

Group RBC(K/uL) HGB(g/dL) HCT(%) MCV(fL) MCH(pg) MCHC(g/dL) PLT(K/uL)
Controls Intact (n=5) 7.7±0.2 14.9±0.3 49.7±0.8 64.8±0.6 19.4±0.4 30.3±0.1 852.4±37.1
CFA (n=5) 7.9±0.1 14.9±0.1 51.1±1.3 62.6±0.4 17.1±0.4 27.3±0.6 1,059.2±272.3
Acupuncture CLL-A (n=5) 8.0±0.2 14.7±0.3 50.4±1.2 59.3±0.4 16.8±0.4 28.3±0.7 1,211.6±225.2
CLL-B (n=5) 8.1±0.2 15.0±0.3 52.1±0.6 62.8±2.0 16.9±2.0 30.1±0.6 903.8±69.3
CLL-C (n=5) 8.1±0.1 15.2±0.2 51.7±1.3 63.5±0.4 18.7±0.4 29.4±0.3 996.0±47.2

Values are expressed mean±SE. A description of controls and acupuncture groups refer to Table 1.