J Korean Acupunct Moxib Soc Search

CLOSE


J Acupunct Res > Volume 30(4); 2013 > Article
Lee, Lee, Lee, Lee, Lee, Shin, and Kim: The Effects of Eucommiae Cortex Pharmacopuncture Injected at Sinsu(BL23) on Neuropathic Pain in Rats
See the Original "".

Abstract

Objectives :

The purpose of this study is to examine whether Eucommiae Cortex pharmacopuncture may affect to the neuropathic pain in a rat model.

Methods :

To produce the model of neuropathic pain, under isoflurane 2.5 % anesthesia, underwent tight ligation by 6.0 silk thread and transection of the tibial and sural nerves, leaving the common peroneal nerve intact. After neuropathic surgery, the author examined if the exhibited the behavioral sign of allodynia. The allodynia was assessed by stimulating the plantar with Dynamic Plantar Aesthesiometer. Three days after the neuropathic surgery, Eucommiae Cortex pharmacopuncture was injected at Sinsu(BL23) once every week for 6 weeks. After that, the author examined the withdrawal response of neuropathic rats’ leg by Dynamic Plantar Aesthesiometer.
And also the author examined Bax, Bcl-2, Bax/Bcl-2 ratio in the spinal cord of neuropathic rats and the change of WBC, RBC, HGB, HCT count in the blood of neuropathic rats.

Results :

  1. The Eucommiae Cortex pharmacopuncture decreased the withdrawal response of mechanical allodynia that assessed with Dynamic Plantar Aesthesiometer in EC2-BL23 group as compared with control group.

  2. The Eucommiae Cortex pharmacopuncture decreased Bax/Bcl-2 ratio in EC1-BL23, EC2-BL23 group. But The Eucommiae Cortex pharmacopuncture injected at Sinsu(BL23) didn’t change Bax, Bcl-2 expression level in the all group.

  3. The Eucommiae Cortex pharmacopuncture decreased WBC count in EC1-BL23, EC2-BL23 group.

Conclusions :

We have noticed that Eucommiae Cortex pharmacopuncture decreased mechanical allodynia in the model of neuropathic pain compared with the control group. Bax/Bcl-2 ratio in spinal cord of that group was also decreased compared with the control group. This study can be used as a basic resource on a study and a treatment of neuropathic pain.

Fig. 1.
Effects of Pharmacopuncuture(Eucommiae Cortex) at BL23 Acupoint on the Withdrawal React Time in Neuropathic Pain Rats
Control : neuropathic pain induced and no treatment.
Saline : neuropathic pain induced and saline injection.
EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.
EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.
Results are shown as mean±SE.
* : p<0.05, ** : p<0.001, as compared with the corresponding data of control group.
acupunct-30-4-69-7-f1.gif
Fig. 2.
Effects of pharmacopuncuture(Eucommiae Cortex) at BL23 acupoint on the withdrawal force intensity in neuropathic pain rats
Control : neuropathic pain induced and no treatment.
Saline : neuropathic pain induced and saline injection.
EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.
EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.
Results are shown as mean±SE.
* : p<0.05, ** : p<0.001, as compared with the corresponding data of control group.
acupunct-30-4-69-7-f2.gif
Fig. 3.
Effects of pharmacopuncture(Eucommiae Cortex) at BL23 acupoint on the intensity of bax mRNA in neuropathic pain rats
Control : neuropathic pain induced and no treatment.
Saline : neuropathic pain induced and saline injection.
EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.
EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.
Results are shown as mean±SE.
acupunct-30-4-69-7-f3.gif
Fig. 4.
Effects of pharmacopuncture(Eucommiae Cortex) at BL23 acupoint on the intensity of bcl-2 mRNA in neuropathic pain rats
Control : neuropathic pain induced and no treatment.
Saline : neuropathic pain induced and saline injection.
EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.
EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.
Results are shown as mean±SE.
acupunct-30-4-69-7-f4.gif
Fig. 5.
Effects of pharmacopuncture(Eucommiae Cortex) at BL23 acupoint on the bax/bcl-2 ratio in neuropathic pain rats
Control : neuropathic pain induced and no treatment.
Saline : neuropathic pain induced and saline injection.
EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.
EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.
Results are shown as mean±SE.
* : p<0.05, ** : p<0.001, as compared with the corresponding data of control group.
acupunct-30-4-69-7-f5.gif
Fig. 6.
Effects of pharmacopuncture(Eucommiae Cortex) at BL23 acupoint on the WBC, RBC, HGB, HCT in hematology of neuropathic pain rats
Control : neuropathic pain induced and no treatment.
Saline : neuropathic pain induced and saline injection.
EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.
EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.
Results are shown as mean±SE.
* : p<0.05, as compared with the corresponding data of control group.
acupunct-30-4-69-7-f6.gif
Table 1.
Effects of Pharmacopuncuture(Eucommiae Cortex) at BL23 Acupoint on the Withdrawal React Time in Neuropathic Pain Rats (%)
Weeks 1 2 3 4 5 6
Group
  Control 100.±0.00 122.6±21.20 128.3±24.81 102.1±10.94 173.7±21.46 176.3±47.93
  Saline 100.±0.00 141.9±47.06 76.6±15.33 77.4±13.91 202.7±51.36 144.7±58.31
  EC-1 100.±0.00 95.6±24.42 110.2±42.84 69.5±14.32 139.6±40.45 131.4±45.71
  EC-2 100.±0.00 66.3±15.47 54.8±9.05* 34.8±7.00** 64.9±13.68* 65.5±10.02*

Pharmacopuncture was treated on the rats for 6 weeks.

Results are shown as means±SE.

*: p<0.05,

**: p<0.001, as compared with the corresponding data of control group.

Table 2.
Effects of Pharmacopuncuture(Eucommiae Cortex) at BL23 Acupoint on the Withdrawal Force Intensity in Neuropathic Pain Rats (%)
Weeks 1 2 3 4 5 6
Group
  Control 100.±0.00 122.0±20.69 127.5±24.27 102.0±10.83 160.0±22.94 169.6±43.80
  Saline 100.±0.00 130.9±36.52 77.1±15.08 77.8±13.69 169.8±29.53 122.5±34.39
  EC-1 100.±0.00 95.5±23.87 109.5±41.67 70.0±14.21 138.4±39.51 130.3±44.53
  EC-2 100.±0.00 66.9±15.14* 55.5±8.93* 35.9±6.86** 65.5±13.34* 66.1±9.80*

Pharmacopuncture was treated on the rats for 6 weeks.

Results are shown as means±SE.

*: p<0.05,

**: p<0.001 as compared with the corresponding data of control group.

Table 3.
Effects of Pharmacopuncture(Eucommiae Cortex) at BL23 Acupoint on the WBC, RBC, HGB, HCT in Hematology of Neuropathic Pain Rats
Group Control Saline EC-1 EC-2
Group
  WBC(K/ul) 6.5±0.52 5.9±0.41 4.7±0.48* 4.6±0.22*
  RBC(M/ul) 8.5±0.13 8.6±0.19 8.7±0.15 8.7±0.17
  HGB(g/dl) 15.4±0.33 14.8±0.45 16.0±0.35 14.9±0.22
  HCT(%) 55.1±1.04 53.9±2.31 55.5±1.62 53.2±0.46

Control : neuropathic pain induced and no treatment. Saline : neuropathic pain induced and saline injection.

EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.

EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.

Results are shown as mean±SE.

*: p <0.05 as compared with the corresponding data of control group.

VI. References

1. Lee BH, Sohn JH, Park YG. Effectiveness of Opioids in the Management of Neuropathic Pain. The Journal of Korean Brain Society. 2001;1(1):53–64.

2. Mitchell SW. Injuries of Nerve and Their Consequences. Philadelphia: JB Lippincott. 1872:252.

3. Commitee of Krean Acupuncture & Moxibustion Textbook Publisher. The Acupuncture and Moxibustion Medicine. Jipmoondang. 2012:16230–54, 617.

4. Korea institute of herbal acupuncture. Herbal Acupuncture treatment guidelines. Korea institute of herbal acupuncture. 2000:13–62.

5. Committee of Korean Textbook Publisher. Herbology. Seoul: Yeonglimsa. 2006:605–6.

6. Hong ND, Rho YS, Kim JW, Won DH, Kim NJ, Cho BS. Studies on the General Pharmacological Activities of Eucommia ulmoides Oliver. Kor J Pharmacogn. 1988;19(2):102–10.

7. Kim BJ. Studies on the analgesic and anti-inflammatory effects by eucommiae cortex extract[dissertation]. Seoul: Kyung Hee Univ.. 2007:Korean.

8. Kim YJ, Kim SH. The effects of Eucommiae Cortex & Eucommia ulmoides Oliv on immune system. Journal of Herbology. 1994;9(1):99–113.
crossref
9. Han SH. Effects of Cortex Eucommiae drink water from cholesterol-induced on lipid metabolism in rats. Korean J Orient Int Med. 2000;21(3):461–5.

10. Lee BH, Baik EJ, Lee SH, Moon CH. Injuries of different distal sciatic nerve branches differentially produce neuropathic pain in rats. In: Abstracts-8th world congress on pain; 1996;27.

11. Koh HK. Transpositional acupoints of the rat. The Journal of Korean Acupuncture & Moxibustion Society. 1999;16(3):115–22.

12. Merskey H, Bogduk N. Classification of chronic pain. Seattle: IASP Press. 1994:

13. Yoon DM. Neuropathic Pain. J Korean Pain Soc. 1995;8(2):219–24.
crossref
14. Moon DE. The Mechanism of Neuropathic Pain. J Korean Pain Soc. 2002;12(1):32–7.
crossref
15. Wegert S, Ossipov MH, Nichols ML, et al. Differential activities of intrathecal MK-801 of morphine to alter responses to thermal and mechanical stimuli in normal or nerve-injured rats. Pain. 1997;71(1):57–64.
crossref pmid
16. Christensen MD, Everhart AW, Pickelman JT, Hulsebosch CE. Mechanical and thermal allodynia in chronic central pain following spinal cord injury. Pain. 1996;68(1):97–107.
crossref pmid
17. Wall PD, Devor M, Inbal R, et al. Autotomy following peripheral nerve lesions: experimental anaesthesia dolorosa. Pain. 1979;7(2):103–13.
crossref pmid
18. Byun BH, Sabina Lim, Lee SW. Acupuncture treatment of Sciatica. The Journal of Jeahan Oriental Medical Academy. 1999;4(1):558–65.

19. An MS, Jeong HY. Study on the Yo Bae Byoung Hoo of the Je Bung Won Hu Ron. Korean J Oriental Physiology & Pathology. 2003;17(3):624–8.

20. Commitee of Korean Acupuncture & Moxibustion Textbook Publisher. Acupuncture & Moxibustion(Sang). Paju: Jipmoondang. 2008:136–7.

21. Jang SJ, Yook TH. The literature study on Sinsu (BL23). The Journal of Korean Acupuncture & Moxibustion Society. 1998;15(1):160.

22. Korean Pharmacopuncture Institute. Pharmacopuncturology. Elsevier. Korea: 2008:3:6–8.

23. Oh HS, Kim HC, Lee SI, Ahn DK. Effects of Eucommiae Cortex and Folium on the Ovariectomized Rat as the Model of Postmenopaual Osteoporosis. Kor J of Herbology. 1995;10(1):59–68.

24. Lee JA, Noh SH, Ann DK, Choi HY. Effects of the Eucommiae Cortex and Chanenomelis Fructus on the Aged Ovariectomized Rat of Postmenopausal Osteoporosis. Kor J of Herbology. 2001;16(1):201–6.

25. Cho JH, Kim KS, Cha JD, et al. Effect of Eucommiae Cortex on Hind Limb Muscle Atrophy of Sciatic Nerve Transectioned Rats. Korean J Oriental Physiology & Pathology. 2008;22(6):1454–61.

26. Nirogi Ramakrishna, Goura Venkatesh, Shanmuganathan Dhanalakshmi, Jayarajan Pradeep, Abraham Renny. Comparison of manual and automated filaments for evaluation of neuropathic pain behavior in rats. Journal of Pharmacological and Toxicological Methods. 2012;66(1):8–13.
crossref pmid
27. Condorelli G, Morisco C, Stassi G, et al. Increased cardiomyocyte apoptosis and changes in proapoptotic and antiapoptotic genes Bax and Bcl-2 during left ventricular adaptations to chronic pressure overload in the rat. Circulation. 1999;99(23):3071–8.
crossref pmid
28. De Novellis V, Siniscalco D, Galderisi U, et al. Blockade of glutamate mGlu5 receptors in a rat model of neuropathic pain prevents early over-expression of pro-apoptic genes and morphological change in dorsal horn lamina II. Neuropharmacology. 2004;46(4):468–79.
crossref pmid
29. Kim YY. Differential expression of Bcl-2, Bax and heat shock proteins in the spinal cord of rat with neuropathic pain [dissertation]. Seoul: Seoul National Univ.. 2003:English.



Article and Issues
For this Journal
For Authors
Ethics
Submit Manuscript
Editorial Office
Gil Korean Medical Hospital, Gachon University
Keunumul-Ro, Chung-Ku, Inchoen 22138, Korea
Tel: +82-70-7606-6353,4    Fax: +82-32-232-3334    E-mail: jared@e-jar.org                

Copyright © 2021 by Korean Acupuncture & Moxibustion Medicine Society. All rights reserved.

Developed in M2PI

Close layer
prev next