The Effects of Sa-Am Spleen-tonifying Acupuncture on Radial Pulse in Healthy Human Subjects

Article information

J Korean Acupunct Moxib Soc. 2013;30(4):1-14
Publication date (electronic) : 2013 September 20
doi : https://doi.org/10.13045/acupunct.2013017
Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Daejeon University
*Corresponding author : Department of Korean Acupuncture & Moxibustion Medicine, Cheonan Oriental Hospital of Daejeon University, 621, Dujeong-dong, Cheonan-si, Chungcheongnam-do, 331-958, Republic of Korea, Tel : +82-41-521-7578 E-mail : lh2000@dju.kr
Received 2013 July 19; Revised 2013 August 24; Accepted 2013 August 24.

Abstract

Objectives :

The purpose of this study is to investigate the effects of Sa-Am spleen-tonifying acupuncture on radial pulse in healthy human subjects.

Methods :

Forty healthy human subjects participated in this study, divided into acupuncture group and control group. Radial pulse was measured by 3 dimensional pulse imaging system(DMP-3000) before, right after, 30 minutes after and 60 minutes after acupuncture in the acupuncture group. The subjects in the control group didn’t received acupuncture but took a rest and then the radial pulse was measured at the same time points as the acupuncture group. The parameters were analyzed by gender, measuring location, and measuring time point. However the time related parameters exceptionally were analyzed without distinction of measuring location.

Results :

  1. T, variance of period, T2/T, T4/T, (T-T4)/T, T4/(T-T4), and W significantly changed after acupuncture.

  2. Pressure, amplitude of H1, amplitude of H2, Amplitude of H4 and pulse energy significantly changed after acupuncture.

  3. Pulse area, systolic pulse area and diastolic pulse area significantly changed after acupuncture.

  4. Elasticity, AIx and AIx/HR significantly changed after acupuncture.

Conclusions :

The effect of Sa-Am spleen-tonifying acupuncture in healthy human may be observed on time, amplitude, pulse area and augmentation index. Further studies on the effects of Sa-Am acupuncture using radial pulse are needed.

Fig. 1.

Radial pulse waveforms obtained with 5 levels of applied pressure

Scale on y-axis means div(digital value for pressure).

Fig. 2.

Normal radial pulse wave form

Fig. 3.

Effect of Sa-Am spleen tonifying acupuncture on pulse period

Healthy subjects were given Sa-Am spleen tonifying acupuncture.

Radial pulse was measured using 3 dimensional pulse imaging system before, right after, 30 minutes after and 60 minutes after acupuncture and pulse period was analyzed.

Data were expressed as mean ± SD(n=60).

□ : control group, : acupuncture group.

* : p<0.05 compared to control group by student t-test.

Fig. 4.

Effect of Sa-Am spleen tonifying acupuncture on pulse period

Healthy subjects were given Sa-Am spleen tonifying acupuncture.

Radial pulse was measured using 3 dimensional pulse imaging system before, right after, 30 minutes after and 60 minutes after acupuncture and variance of pulse period was analyzed.

Data were expressed as mean ± SD(n=60).

□ : control group, : acupuncture group.

* : p<0.05 compared to control group by student t-test.

Fig. 5.

Effect of Sa-Am spleen tonifying acupuncture on T2/T of radial pulse wave

Healthy subjects were given Sa-Am spleen tonifying acupuncture. Radial pulse was measured using 3 dimensional pulse imaging system before, right after, 30 minutes after and 60 minutes after acupuncture and T2/T of representative pulse was analyzed.

Data were expressed as mean ± SD(n=60).

□ : control group, : acupuncture group.

* : p<0.05 compared to control group by student t-test.

Fig. 6.

Effect of Sa-Am spleen tonifying acupuncture on T4/T of radial pulse wave

Healthy subjects were given Sa-Am spleen tonifying acupuncture. Radial pulse was measured using 3 dimensional pulse imaging system before, right after, 30 minutes after and 60 minutes after acupuncture and T4/T of representative pulse was analyzed.

Data were expressed as mean ± SD(n=60).

□ : control group, : acupuncture group.

* : p<0.05 compared to control group by student t-test.

Fig. 7.

Effect of Sa-Am spleen tonifying acupunctur on (T-T4)/T of radial pulse wave

Healthy subjects were given Sa-Am spleen tonifying acupuncture. Radial pulse was measured using 3 dimensional pulse imaging system before, right after, 30 minutes after and 60 minutes after acupuncture and (T-T4)/T of representative pulse was analyzed.

Data were expressed as mean ± SD (n=60).

□ : control group, : acupuncture group.

* : p<0.05 compared to control group by student t-test.

Fig. 8.

Effect of Sa-Am spleen tonifying acupunctur on T4/(T-T4) of radial pulse

Healthy subjects were given Sa-Am spleen tonifying acupunctu. Radial pulse was measured using 3 dimensional pulse imaging system before, right after, 30 minutes after and 60 minutes after acupuncture and T4/(T-T4) of representative pulse was analyzed.

Data were expressed as mean ± SD(n=60).

□ : control group, : acupuncture group.

* : p<0.05 compared to control group by Student t-test.

Fig. 9.

Effect of Sa-Am spleen tonifying acupunctur on W of radial pulse

Healthy subjects were given Sa-Am spleen tonifying acupunctur. Radial pulse was measured using 3 dimensional pulse imaging system before, right after, 30 minutes after and 60 minutes after acupuncture and W of representative pulse was analyzed.

Data were expressed as mean ± SD(n=60).

□ : control group, : acupuncture group.

* : p<0.05 compared to control group by student t-test.

Fig. 10.

Effect of Sa-Am spleen tonifying acupunctur on the pressure applied for acquisition of tepresentative pulse

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi..

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 11.

Effect of Sa-Am spleen tonifying acupunctur on on H1 of radial pulse

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 12.

Effect of Sa-Am spleen tonifying acupunctur on H2 of radial pulse

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 13.

Effect of Sa-Am spleen tonifying acupunctur on H4 of radial pulse

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 14.

Effect of Sa-Am spleen tonifying acupunctur on pulse energy

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 15.

Effect of Sa-Am spleen tonifying acupunctur on pulse area

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test..

Fig. 16.

Effect of Sa-Am spleen tonifying acupunctur on systolic pulse area

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 17.

Effect of Sa-Am spleen tonifying acupunctur on diastolic pulse area

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 18.

Effect of Sa-Am spleen tonifying acupunctur on radial elasticity

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 19.

Effect of Sa-Am spleen tonifying acupunctur on AIx

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi..

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 20.

Effect of Sa-Am spleen tonifying acupunctur on AIx/HR

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.

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Article information Continued

Fig. 1.

Radial pulse waveforms obtained with 5 levels of applied pressure

Scale on y-axis means div(digital value for pressure).

Fig. 2.

Normal radial pulse wave form

Fig. 3.

Effect of Sa-Am spleen tonifying acupuncture on pulse period

Healthy subjects were given Sa-Am spleen tonifying acupuncture.

Radial pulse was measured using 3 dimensional pulse imaging system before, right after, 30 minutes after and 60 minutes after acupuncture and pulse period was analyzed.

Data were expressed as mean ± SD(n=60).

□ : control group, : acupuncture group.

* : p<0.05 compared to control group by student t-test.

Fig. 4.

Effect of Sa-Am spleen tonifying acupuncture on pulse period

Healthy subjects were given Sa-Am spleen tonifying acupuncture.

Radial pulse was measured using 3 dimensional pulse imaging system before, right after, 30 minutes after and 60 minutes after acupuncture and variance of pulse period was analyzed.

Data were expressed as mean ± SD(n=60).

□ : control group, : acupuncture group.

* : p<0.05 compared to control group by student t-test.

Fig. 5.

Effect of Sa-Am spleen tonifying acupuncture on T2/T of radial pulse wave

Healthy subjects were given Sa-Am spleen tonifying acupuncture. Radial pulse was measured using 3 dimensional pulse imaging system before, right after, 30 minutes after and 60 minutes after acupuncture and T2/T of representative pulse was analyzed.

Data were expressed as mean ± SD(n=60).

□ : control group, : acupuncture group.

* : p<0.05 compared to control group by student t-test.

Fig. 6.

Effect of Sa-Am spleen tonifying acupuncture on T4/T of radial pulse wave

Healthy subjects were given Sa-Am spleen tonifying acupuncture. Radial pulse was measured using 3 dimensional pulse imaging system before, right after, 30 minutes after and 60 minutes after acupuncture and T4/T of representative pulse was analyzed.

Data were expressed as mean ± SD(n=60).

□ : control group, : acupuncture group.

* : p<0.05 compared to control group by student t-test.

Fig. 7.

Effect of Sa-Am spleen tonifying acupunctur on (T-T4)/T of radial pulse wave

Healthy subjects were given Sa-Am spleen tonifying acupuncture. Radial pulse was measured using 3 dimensional pulse imaging system before, right after, 30 minutes after and 60 minutes after acupuncture and (T-T4)/T of representative pulse was analyzed.

Data were expressed as mean ± SD (n=60).

□ : control group, : acupuncture group.

* : p<0.05 compared to control group by student t-test.

Fig. 8.

Effect of Sa-Am spleen tonifying acupunctur on T4/(T-T4) of radial pulse

Healthy subjects were given Sa-Am spleen tonifying acupunctu. Radial pulse was measured using 3 dimensional pulse imaging system before, right after, 30 minutes after and 60 minutes after acupuncture and T4/(T-T4) of representative pulse was analyzed.

Data were expressed as mean ± SD(n=60).

□ : control group, : acupuncture group.

* : p<0.05 compared to control group by Student t-test.

Fig. 9.

Effect of Sa-Am spleen tonifying acupunctur on W of radial pulse

Healthy subjects were given Sa-Am spleen tonifying acupunctur. Radial pulse was measured using 3 dimensional pulse imaging system before, right after, 30 minutes after and 60 minutes after acupuncture and W of representative pulse was analyzed.

Data were expressed as mean ± SD(n=60).

□ : control group, : acupuncture group.

* : p<0.05 compared to control group by student t-test.

Fig. 10.

Effect of Sa-Am spleen tonifying acupunctur on the pressure applied for acquisition of tepresentative pulse

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi..

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 11.

Effect of Sa-Am spleen tonifying acupunctur on on H1 of radial pulse

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 12.

Effect of Sa-Am spleen tonifying acupunctur on H2 of radial pulse

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 13.

Effect of Sa-Am spleen tonifying acupunctur on H4 of radial pulse

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 14.

Effect of Sa-Am spleen tonifying acupunctur on pulse energy

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 15.

Effect of Sa-Am spleen tonifying acupunctur on pulse area

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test..

Fig. 16.

Effect of Sa-Am spleen tonifying acupunctur on systolic pulse area

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 17.

Effect of Sa-Am spleen tonifying acupunctur on diastolic pulse area

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 18.

Effect of Sa-Am spleen tonifying acupunctur on radial elasticity

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 19.

Effect of Sa-Am spleen tonifying acupunctur on AIx

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi..

* : p<0.05 compared to control group by Mann Whitney U test.

Fig. 20.

Effect of Sa-Am spleen tonifying acupunctur on AIx/HR

Data were expressed as mean ± SD(n=10).

□ : control group, : acupuncture group.

L1 : left Cun. L2 : left Guan. L3 : left Chi.

R1 : right Cun. R2 : right Guan. R3 : right Chi.

* : p<0.05 compared to control group by Mann Whitney U test.