The Effect of Phaseoli Semen Herbal-acupuncture at KI10 in Lipopolysaccharide Induced Acute Nephritis in Rats

Article information

J Korean Acupunct Moxib Soc. 2013;30(3):61-73
Publication date (electronic) : 2013 June 20
doi : https://doi.org/10.13045/acupunct.2013006
Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Daejeon University
*Corresponding author : Department of Korean Acupuncture & Moxibustion Medicine, Cheonan Oriental Hospital of Daejeon University, 621, Dujeong-dong, Cheonan-si, Chungcheongnam-do, 331-958, Republic of Korea Tel : +82-41-521-7578 E-mail : lh2000@hanmir.com
Received 2013 May 09; Revised 2013 June 01; Accepted 2013 June 03.

Abstract

Objectives :

This study was designed to evaluate the effects of Phaseoli Semen Herbal-acupuncture(PS-HA) at KI10 in acute nephritis induced by lipopolysaccharide(LPS) in rat.

Methods :

The rats were divided into 5 groups, which were control, LPS, PS-HA, NP and saline group. LPS, PS-HA, NP and saline groups were given LPS to induce acute nephritis and control group did not receive LPS. LPS group did not receive any treatment after the onset of acute nephritis. PS-HA, NP and saline group received PS-HA, normal acupuncture, and saline injection at KI10 three times per week, respectively. To evaluate the effect of PS-HA at KI10, the complete blood count, BUN, creatinine, TNF-α, and CINC-1 in serum were measured. To show its effect on renal function, creatinine, and total protein in urine was measured as well as urine output. The level of myeloperoxidase in renal tissue was quantified and complete histology was done in kidney samples obtained from the rats.

Results :

PS-HA group showed a significant reduction in the proportion of WBC and neutrophil, serum BUN, TNF-a, and CINC-1 compared to LPS group. Furthermore, a significant increase in urine output and a decrease in urinary creatinine level, MPO in renal tissue, and number of neutrophils at glomerulus was observed in PS-HA group compared to LPS group

Conclusions :

PS-HA at KI10 was shown to have a significantly effect on treating LPS induced acute nephrits. Therefore, future study is needed to further evaluate the clinical usefulness of PS-HA at KI10 in treating acute nephritis.

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