The Efficacy of Eriobotryae Folium Pharmacopuncture on Local Fat of High Fat Diet Induced Obesity Mice

Article information

J Korean Acupunct Moxib Soc. 2013;30(3):15-25
Publication date (electronic) : 2013 June 20
doi :
1Department of Herbology, College of Oriental Medicine, Daegu Haany University
2Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Daegu Haany University
*Corresponding author : Department of Acupunture & Moxibustion Medicine, Pohang Oriental Medical Hospital of Daegu Haany University, 411, Saecheonnyeon-daero, Nam-gu, Pohang-si, Gyeongsangbuk-do, 790-826, Republic of Korea Tel : 82-54-271-8009 E-mail :
Received 2013 April 15; Revised 2013 May 21; Accepted 2013 May 21.


Objectives :

This study was designed to investigate the anti-obesity effects in local area adipose tissue using by pharmacopuncture of Eriobotryae Folium on mice fed high fat diet.

Methods :

Male ICR mouse were divided into three groups. Normal diet group (N), High-fat diet(HFD) group, HFD+E(pharmacopuncture of the Eriobotryae Folium) group for 8 weeks. HFD+E group was injected in the concentration of 50 mg/kg once a day for 4 weeks and every other day during the next 4 weeks. We measured body weight, food intake, absolute weight and relative weight in the liver and adipose tissue. Also, we analyzed hematological levels and histopathological changes on adipose tissue.

Results :

1. We observed no difference in food intake among three groups.

2. HFD+E group significantly decreased body weight gain and absolute weight and relative weight in the liver and adipose tissue compared with HFD group.

3. Blood AST and ALT were no differences among the experiment groups. However, HFD group was significantly increased higher TG, TC, HDL and LDL than in N group and HDL/LDL ratio was remarkably decreased. On the other hand, HFD+E group was significantly decreased in obesity markers and HDL/LDL ratio was remarkably increased.

4. Levels of adiponectin in serum of HFD+E group was remarkably increased compared with those of HFD group.

5. HFD group was significantly decreased compared with N group in adipocyte number. Compared with the HFD group, HFD+E group was displayed a significant increase by adipocyte number.

Conclusions :

We suggest that pharmacopuncture of the Leaves of Eriobotrya japonica can play the anti-obesity effects in local area adipose tissue.


This research was supported by a grant from Daegu Haany University Kylin Foundation in 2011


1. Son Y, Nam JS, Jang MK, Jung IA, Cho SI, Jung MH. Antiobesity Activity of Vigna nakashimae Extract in High-Fat Diet-Induced Obesity. Biosci Biotechnol Biochem 2013;77(2):332–8.
3. Parsi MA. Obesity and cholangiocarcinoma. World J Gastroenterol 2013;19(4):457–62.
4. Rtveladze K, Marsh T, Barquera S, et al. Obesity prevalence in Mexico: impact on health and economic burden. Public Health Nutr 2013;Feb. 1. :1–7.
5. Fantasia HC. New developments in the pharmacologic treatment of obesity. Nurs Womens Health 2013;17(1):53–62.
6. Ministry of Health and welfare. Obesity rate of South Korea. 2012.
7. Padwal RS, Majumdar SR. Drug treatments for obesity: orlistat, sibutramine, and rimonabant. Lancet 2007;369(9555):71–7.
8. Filippatos TD, Derdemezis CS, Gazi IF, Nakou ES, Mikhailidis DP, Elisaf MS. Orlistat-associated adverse effects and drug interactions : a critical review. Drug Saf 2008;31(1):53–65.
9. Feng XM, Xiong J, Qin H, et al. Fluoxetine induces hepatic lipid accumulation via both promotion of the SREBP1c-related lipogenesis and reduction of lipolysis in primary mouse hepatocytes. CNS Neurosci Ther 2012;18(12):974–80.
10. [Ministry of Food and Drug Safety/Intergrated services of Herb medicine/Information of Herb medicine/DB searching of Herb medicine].
11. Herbal Pharmacology Text Compilation Committee. Herbal Pharmacology. Shin-il Books 2010. p. 295–6.
12. Lee SI. Herbal medicine Seoul: Su-seo-won; 1981. p. 340–1. p. 513–4.
13. Yook CS, Kim SM, Jung JM, Jung MS, Kim JH, Kim SB. Pharmacological action · Component · Clinical Application of Herbal Medicine Seoul: Gye-Chuk company; 1992. p. 885–6.
14. Chen J, Li WJ, Wu JL, Ren BR, Zhang HQ. Hypoglycemic effects of a sesquiterpene glycoside isolated freom leaves of loquat (Eriobotrya japonica(THUNB.) indl.). Phytomedicine 2008;15(1–2):98–102.
15. Hong Y, Lin S, Jing Y, Ashraf M. Variation in contents of total phenolics and flavonoids and antioxidant activities in the leaves of 11 Eriobotrya species. Plant Foods Hum Nutr 2008;63(4):200–4.
16. Ito H, Kobayashi E, Li SH, et al. Antitumor activity of compounds isolated from leaves of Eriobotrya japonica. J Agric Food Chem 2002;50(8):2400–3.
17. Banno N, Akihisa T, Tokuda H, et al. Anti-inflammatiory and antitumor-promoting effect of the triterpene acid from the leaves of Eriobotrya japonica. Biol Pharm Bull 2005;28(10):1995–9.
18. Park CG, Bang KH, Lee SE. Antibacterial Activity from Medicinal plant extracts on the Staphylococcus aureus. Korean joural of medicinal crop science 2001;9(4):251–8.
19. Bae YI, Chung YC, Shim KH. Antimicrobial and antioxidant activities of various solvent extact from different parts of loquat(Eriobotrya japonica Lindl.). Korean J Food Preservation 2002;9(1):97–101.
20. Jeong YS, Jung HK, Youn KS, Kim MS, Hong JS. Hysiological Activities of the Hot Water Extract from Eriobotrya japonica Lindl. J Korean Soc Food Sci Nutr 2009;38(8):977–82.
21. Kim AR, Hwang YK, Lee JJ, Jung HO, Lee MR. Effects of Eriobotrya japonica Lindl. (Loquat) Leaf Ethanol Extract on Cholesterol and Antioxidative Activity in Rats Fed a High-Fat/High-Cholesterol Diet. J Korean Soc Food Sci Nutr 2011;40(5):673–81.
22. Kim MK, Han K, Kwon HS, et al. Normal-weight obesity in Korean adults. Clin Endocrinol Oxf 2013;10.1111/cen.12162.
23. Mi Jung Park. Recent Advances in Regulating Energy Homeostasis and Obesity. Korean Journal of Pediatrics 2005;48(2):126–37.
24. Kim HC. Studies on the effects eriobotrya japonica on the pancreatic endocrine cells in the diabetic rats[dissertation]. Seoul: Dongkuk University; 1993. Korean.
25. Park HS. Effects of Eriobotryae Folium(EF) on Gene Expression in Human HaCaT keratinocyte. 2009. 1. 40.
26. Shih CC, Lin CH, Wu JB. Eriobotrya japonica improves hyperlipidemia and reverses insulin resistance in high-fat-fed mice. Phytotherapy Research 2010;24(12):1769–80.
27. Oh J, Min OJ, Kim HA, Kim YJ, Baek HY, Rhyu DY. Effect of Eriobotrya japonica on Adipogenesis and Body Weight. Journal of the Korean Society for Applied Biological Chemistry 2011;54(3):382–7.
28. Herbal medicene compilation committee of College of Oriental medicine. Herbal medicine Seoul: Yong-lim company; 2004. p. 509–10.
29. Cha JT. Anti-asthmatic acivities of leaces of Eribotrya japonica lindley in a mouse model of allergic asthma. 2012. 37–8.
30. Bjorntorp P. The associations between obesity, adipose tissue distribution and disease. Acta Med Scand 1988;723:121–34.
31. Plaa GL, Charbonneau M. Detection and evaluation of chemically induced liver injury. Principles and Methods of Toxicology New York: In : Hayes AW, ed. Raven Press; 1994. p. 839–70.
32. Park BS. Effect of the Feeding Belly Fat on Plasma Lipids Levels in Rats. Korean J Food Sci Ani Resour 2004;24(2):189–97.
33. Rosenfeld L. Lipoprotein analysis. Arch pathol Lab 1989;113:1101–10.
34. Schulze MB, Rimm EB, Shai I, Rifai N, Hu FB. Relationship Between Adiponectin and Glycemic Control, Blood Lipids, and Inflammatory Markers in Men With Type 2 Diabetes. Diabetes Care 2004;27(7):1680–7.
35. Schulze MB, Shai I, Rimm EB, Li T, Rifai N, Hu FB. Adiponectin and Future Coronary Heart Disease Events Among MenWith Type 2 Diabetes. Diabetes Februrary. 2005;54(2):534–9.
36. Mela David J. Food, diet and abecity. Hanmi medicine 2007. 13–25.
37. Park Jiyoung, Kim Jae Bum. Molecular Insights into Fat Cell Differentiation and Functional Roles of Adipocytokines. Jourmal of Korean society of endocrinology 2002;17(1):1–9.

Article information Continued