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J Acupunct Res > Volume 29(6); 2012 > Article
Published online December 17, 2012.
DOI: https://doi.org/10.13045/kamms.2012084   
Effects of Sujeom Powder Pharmacopuncture Injected at Jung-wan(CV12) on The Caerulein-induced Acute Pancreatitis in the Rat
In Soo Kim, Sang Yun Jeon, Tae San Jeong, Sung Sun Kang, Jae Jun Jo, Young Su Lee
Department of Orienal Medicine, Graduate School of Dongshin University, Korea
*Corresponding author.
Young Su Lee
Tel: 010-2680-4147   Email: hefari92@hanmail.net
Abstract
Objectives : This study was designed to investigate Effects of Sujeom powder (SJP) pharmacopuncture Injected at Jung-wan(CV12) in rats with caerulein-induced acute pancreatitis(AP).
Methods : We examined changes of organ weight, histology, immunohistochemistry and gene expression of cycolooxygenase 2(COX-2) in the pancreas. Twenty adult male Sprague-Dawley rats were divided into four groups as follow: normal(Nor), caerulein-induced(Con), caerulein+SJP pharmacopuncture 0.2mL injected at Jung-wan(CV12)(SA), and caerulein+SJP pharmacopuncture 0.8mL injected at Jung-wan(CV12)(SB) groups. Pancreatic tissues of rats from all groups were removed for histological observation and light microscopic examination. Interleukin-6(IL-6) levels were determined spectrophotometrically.
Results : The ratio of pancreas/body weights was significantly(p<0.05) increased in the Con, the SA and the SB compared with the Nor, but was slightly decreased in the SA and in the SB groups compared with the Con. Caerulein administration has significantly(p<0.05) increased in the levels of amylase, but the SA, the SB significantly(p<0.05) decreased in the levels of these enzyme. The levels of amylase were increased significantly with caerulein administration, but were inhibited significantly in the SA and in the SB groups. Interleukin-6(IL-6) levels were significantly(p<005) increased in all groups compared with the Nor, especially in the SB. were significantly increased. The levels of Tumor necrosis factor(TNF)-α levels were significantly increased in all groups compared with the Nor. In the conclusion, the datum of IL-6 and TNF-α are suggested that the inflamation was still existed actively at a point of measurement(24 hours later). The COX-2 positive materials are observed in the pancreas from the Con, but these positive materials are decreased in the SJP pharmacopuncture at Jung-wan(CV12) treatment group. Conclusion : SJP pharmacopuncture injected at Jung-wan(CV12) is potentially capable of limiting pancreatic damage during AP by restoring the fine structure of acinar cells and tissues. Therefore we can say that SJP pharmacopuncture Injected at Jung-wan(CV12) may have beneficial effects in the treatment of caerulein-induded AP. Further studies about the adequate amount of the SJP pharmacopuncture and about more effective route of administration is still required .
Keywords: Sujeom powder(SJP), pharmacopuncture, caerulein, pancreatitis, amylase


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