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J Acupunct Res > Volume 32(4); 2015 > Article
Yu, Kim, Lee, Lee, and Yook: The Effects of Lonicerae Flos, Forsythiae Fluctus and Hwangryunhaedok Decoction Pharmacopuncture on Atopic Dermatitis in NC/Nga Mice※

Abstract

Objectives:

Atopic dermatitis is a chronic inflammatory skin disease characterized by pruritic and erythematous skin lesions. The purpose of this study was to investigate the suppressive effects of Lonicerae Flos, Forsythiae Fluctus and Hwangryunhaedok Decoction Pharmacop-uncture on the development of atopic dermatitis-like skin lesions in NC/Nga Mice.

Methods:

The Atopic Dermatitis was induced by biostir AD on the mice’s back skin. Experimental groups were divided into three including LFP (Lonicerae Flos Pharmacopuncture, EtOH extract), FFP (Forsythiae Fluctus Pharmacopuncture, EtOH extract) and HHP (Hwangryunhaedok Decoction Pharmacopuncture, Hydrodistillation extract). Every second day, the mice of three groups were treated with 0.1 ㎖ of pharmacopuncture using a syringe at right and left acupoints (BL13), alternatively. On the control group, normal saline was used instead of pharmacopuncture. Subsequently optical observation with a handscope, a clinical skin score, Tissue(general/immune) mast cell, Serum IgE level, Serum histamine level, and Serum lymphokine(IL-2, IL-4, IFN-γγ) were measured.

Results:

FFP and HHP decreased the clinical skin score, the total cell number of mast cells, and the Serum total IgE level and Serum histamine level. In Serum lymphokine levels, all groups were decreased to the IL-4 level, LFP and FFP were increased to the IL-2 level, and LFP was increased to the IFN-γ level.

Conclusions:

From the above results, Forsythiae Fluctus Pharmacopuncture (EtOH extract) and Hwangryunhaedok Decoction Pharmacopuncture (Hydrodistillation extract) exerted anti-allergic and anti-inflammatory effects, suggesting a promising agent for improving atopic dermatitis related symptoms.

Fig. 1
Handscopic observation of atopic dermatitis like skin lesions for 3 weeks
1a. Normal mice; 1b∼d, atopic dermatitis NC/Nga mice model induced by biostir AD for 3 weeks; scaling/dryness and edema(1b), scaling/dryness, and erythema/hemorrhage(1c), scaling/dryness, erythema/hemorrhage and excoriation/erosion(1d).
acup-32-4-119f1.gif
Fig. 2
Effects of pharmacopuncture treatment for 2 weeks on handscopic observation of atopic dermatitis
2a. control group; 2b. LFP group; 2c. FFP group; 2d. HHP group.
Handscopic features of FFP(2c) group were observed lower clinical skin scores graded on the skin lesions(scaling/dryness) compared to LFP(2b) and HHP(2d) groups.
acup-32-4-119f2.gif
Fig. 3
Effects of pharmacopuncture treatment for 3 weeks on handscopic observation of atopic dermatitis
3a. control group; 3b. LFP group; 3c. FFP group; 3d. HHP group.
Handscopic features of FFP(3c) group and HHP(3d) group were observed lower clinical skin scores graded on the skin lesions(scaling/dryness) compared to control group(3a).
acup-32-4-119f3.gif
Fig. 4
Histological section of atopic dermatitis after pharmacopuncture treatment for 3 weeks (toluidin blue, × 200)
4a. control group; 4b. LFP group; 4c. FFP group; 4d. HHP group.
Distribution of mast cells in the skin of FFP(4c) and HHP (4d) groups were more decreased compare to that of control(4a) group. Degranulated mast cells were more increase in control group(4a) compare to the other groups(4b∼d).
acup-32-4-119f4.gif
Fig. 5
Numerical changes of mast cells of atopic dertitis after pharmacopuncture treatment for 3 weeks
The number of mast cells in skin was more decreased FP and HHP groups compare to control group. Valuse are mean±standard deviation. *; p-value<0.05.
acup-32-4-119f5.gif
Fig. 6
Effects of pharmacopuncture treatment of LFP, FFP, HHP for 3 weeks on the serum IgE level atopic dermatitis
Values are mean±standard deviation.
*; p-value<0.05.
acup-32-4-119f6.gif
Fig. 7
Effects of pharmacopuncture treatment of LFP, FFP, HHP for 3 weeks on the serum histamine level of atopic dermatitis
Values are mean±standard deviation.
*; p-value<0.01.
acup-32-4-119f7.jpg
Fig. 8
Effects of pharmacopuncture treatment of LFP, FFP, HHP for 3 weeks on the serum IL-2 level of atopic dermatitis
Values are mean±standard deviation.
*; p-value<0.05.
acup-32-4-119f8.jpg
Fig. 9
Effects of pharmacopuncture treatment of LFP, FFP, HHP for 3 weeks on the serum IL-4 level of atopic dermatitis
Values are mean±standard deviation.
*; p-value<0.05.
acup-32-4-119f9.gif
Fig. 10
Effects of pharmacopuncture treatment of LFP, FFP, HHP for 3 weeks on the serum IFN-γ level of atopic dermatitis
Values are mean±standard deviation.
*; p-value<0.01.
acup-32-4-119f10.gif
Table 1
Effects of pharmacopunctures on clinical skin score of Atopic Dermatitis induced by biostir AD in NC/Nga mouse
Groups Clinical Skin Score
1 week 2 week 3 week
Control 6.5±0.3 6.2±0.5 5.4±0.3
LFP 6.4±0.5 5.6±0.4 5.2±0.4
FFP 4.2±0.2* 3.5±0.3* 2.8±0.2*
HHP 6.2±0.4 4.8±0.6 4.0±0.2*

a) Control group, AD+Saline pharmacopuncture;

LFP group, AD+Lonicerae Flos EtOH extract pharmacopuncture;

FFP group, AD+Forsythiae Fructus EtOH extract pharmacopuncture;

HHP group, AD+Hwangryunhaedok Decoction pharmacopuncture.

b) Values are mean±standard deviation.

* p-value<0.05.

c) Total clinical skin score for atopic dermatitis like lesions of NC/Nga mice was defined as the sum of individual scores graded as 0(none), 1(mild), 2(moderate), 3(severe) for each 4 signs and symptoms(erythema/hemorrhage, scaling/dryness, excoriation/erosion) on the back skin.

Table 2
Effects of pharmacopunctures on numerical changes of mast cells of Atopic Dermatitis induced by biostir AD in NC/Nga mouse
Mast cells(No.)
Control 32.9±5.4
LFP 23.4±4.5
FFP 13.8±2.3*
HHP 16.2±2.6*

a) Control group, AD+Saline pharmacopuncture;

LFP group, AD+Lonicerae Flos EtOH extract pharmacopuncture;

FFP group, AD+Forsythiae Fructus EtOH extract pharmacopuncture;

HHP group, AD+Hwangryunhaedok Decoction pharmacopuncture.

b) Values are mean±standard deviation.

* p-value<0.01.

Table 3
Effects of pharmacopunctures on the serum IgE level of Atopic Dermatitis induced by biostir AD in NC/Nga mouse
IgE(ng/ml)
Control 423.9±7.5
LFP 636.3±9.2
FFP 354.0±5.2*
HHP 388.7±6.3*

a) Control group, AD+Saline pharmacopuncture;

LFP group, AD+Lonicerae Flos EtOH extract pharmacopuncture;

FFP group, AD+Forsythiae Fructus EtOH extract pharmacopuncture;

HHP group, AD+Hwangryunhaedok Decoction pharmacopuncture.

b) Values are mean±standard deviation.

* p-value<0.05.

Table 4
Effects of pharmacopunctures on the serum histamine level of Atopic Dermatitis induced by biostir AD in NC/Nga mouse
histamine(nM)
Control 62.3±2.6
LFP 55.5±4.3
FFP 33.6±3.2*
HHP 12.9±2.3*

a) Control group, AD+Saline pharmacopuncture;

LFP group, AD+Lonicerae Flos EtOH extract pharmacopuncture;

FFP group, AD+Forsythiae Fructus EtOH extract pharmacopuncture;

HHP group, AD+Hwangryunhaedok Decoction pharmacopuncture.

b) Values are mean±standard deviation.

* p-value<0.01.

Table 5
Effects of pharmacopunctures on the serum IL-2 level of Atopic Dermatitis induced by biostir AD in NC/Nga mouse
IL-2(pg/ml)
Control 75.1±3.0
LFP 93.4±0.8*
FFP 82.3±3.6*
HHP 51.6±0.5

a) Control group, AD+Saline pharmacopuncture;

LFP group, AD+Lonicerae Flos EtOH extract pharmacopuncture;

FFP group, AD+Forsythiae Fructus EtOH extract pharmacopuncture;

HHP group, AD+Hwangryunhaedok Decoction pharmacopuncture.

b) Values are mean±standard deviation.

* p-value<0.05.

Table 6
Effects of pharmacopunctures on the serum IL-4 level of Atopic Dermatitis induced by biostir AD in NC/Nga mouse
IL-4(pg/ml)
Control 117.2±0.0
LFP 81.9±0.2*
FFP 103.2±1.7*
HHP 99.3±1.0*

a) Control group, AD+Saline pharmacopuncture;

LFP group, AD+Lonicerae Flos EtOH extract pharmacopuncture;

FFP group, AD+Forsythiae Fructus EtOH extract pharmacopuncture;

HHP group, AD+Hwangryunhaedok Decoction pharmacopuncture.

b) Values are mean±standard deviation.

* p-value<0.05.

Table 7
Effects of pharmacopunctures on the serum IFN-γ level of Atopic Dermatitis induced by biostir AD in NC/Nga mouse
IFN-γ (pg/ml)
Control 257.6±3.1
LFP 428.6±1.2*
FFP 260.0±12.7
HHP 193.3±13.7

a) Control group, AD+Saline pharmacopuncture;

LFP group, AD+Lonicerae Flos EtOH extract pharmacopuncture;

FFP group, AD+Forsythiae Fructus EtOH extract pharmacopuncture;

HHP group, AD+Hwangryunhaedok Decoction pharmacopuncture.

b) Values are mean±standard deviation.

* p-value<0.01.

VI.
VI.

References

1. Lee JH, Kim KH, Kim MN, et al. Report from ADRG: A Study on the Diagnostic Criteria of Korean Atopic Dermatitis. Korean J Dermatology. 2006;44(8):907–13.

2. Kim KH, Park CW, Eun HC, Cho SH. Atopic dermatitis. Dermatology. 5th ed. Seoul: Ryo Mon Gak. 2008:170–8.
crossref
3. Park JH, Choi IH. The Effect of Herbal-Acupuncture Using Ursi Fel into Zusanli(ST36) to Recover Function of Stratum Corneum on Mice Model after Atopic Dermatitis Elicitation. J Korean Oriental Med. 2005;26(3):13–26.

4. Seo BJ. Effect of Bee Venom Acupuncture on Trimellitic Anhydride-induced Atopic Dermatitis in Mice [dissertation]. Seoul: Kyunghee Univ, 2010. Korean.

5. Kim MC. The Effects of Soyeom and Rehmanniae radix Pharmacopuncture on Atopic Dermatitis in NC/Nga Mice [dissertation]. Jeounju: Woosuk Univ, 2011. Korean.

6. The Acupuncture and Moxibustion Medicine textbook compilation committee. The Acupuncture and Moxibustion Medicine(上). Paju: Jipmoondang. 2008:130–1.

7. Vestergaard C, Yoneyama H, Matsushima K. The NC/Nga mouse: a model for atopic der matitis. Molecular Medecine Today. 2000;6(5):209–10.
crossref
8. Youn DH, Choi GJ, Chae WS, Na CS. Effects of Cordyceps militaris Mycelia(CMM) herbal acupuncture at BL13, LU4 on airway smooth muscle, airway inflammation, IgE and Interleukin-4 in mouse model of allergic bronchial asthma. Korean J Acupunct. 2004;21(2):111–23.

9. Jun HK, Chung JS, Seo YM, Park SK, Lee YW, Kim DH. Treatment of Canine Tracheal Collapse by Injection-Acupuncture and Herbal Medicine. J Vet Clin. 2007;24(3):419–21.

10. Yamamoto M, Haruna T, Ueda C, et al. Contribution of itch-associated scratch behavior to the development of skin lesions in Dermatophagoides farinae-induced dermatitis model in NC/Nga mice. Arch Dermatol Res. 2009;301(10):739–46.
crossref pmid
11. Enavall E, Perlmann P. Enzyme-linked immuno-sorbent assay III, Quantitation of specific antibodies of enzyme labelled anti-Immunoglobulin in antigen-coated tubes. J Immunol. 1972;109:129–35.
pmid
12. Kim JJ, Yang SW, Son NW, Ann KS. Anti-inflammatory action by Gamisangryosamultang and the effect of Ziyang-Go on atopic dermatitis-like lesion and pruritus in NC/Nga mice. KJOMPP. 2003;17(2):428–35.

13. Hanifin JM, Rajka G. Diagnostic features of atopic dermatitis. Acta Dermato-Venereologica. Supplementum. 1980;92:44–7.

14. Leung DY, Boguniewicz M, Howell MD, Nomura I, Hamid QA. New insights into atopic dermatitis. J Clin Invest. 2004;113(7):651–7.
crossref pmid pmc
15. The Korean Academy of Pediatric Allergy and Respiratory Disease. Pediatric Allergy and Respiratory disease. Seoul: Koonja publishing company. 2005:145–73.

16. Kim DH, Kang KH, Kim KW, Yoo IY. Management of Children with Atopic Dermatitis. AARD. 2008;18(2):148–57.

17. Kim SC, An KJ, Han SG, et al. Clinico-Epidemiologic study on the Abuse, Misuse, and Adverse Effects of Topical Dermatologic Drugs. Korean J Dermatol. 2003;41(9):1129–35.

18. Lee SJ, Lee MJ, Jung JE, et al. Antimicrobial Effects of Fermented Coptidis rhizoma and Lonicerae Flos against pathogen. J Korean Med Obes Res. 2011;11(1):35–46.

19. Chung KJ, Jung HJ, Jung SK, Rhee HK. Lonicerae Flos contributes to the chemotaxis of eosinophils and secretion of cytokines in A549 human epithelial cells. Korean J Orient Int Med. 2005;26(1):129–42.

20. Lee DE, Lee JR, Kim YW. Inhibition of Lipopolysaccharide-Inducible Nitric Oxide Synthase, TNF-α, IL-1β and COX-2 Expression by Flower and Whole Plant of Lonicera japonica. KJOMPP. 2005;19(2):481–9.

21. Park HS. The Effects of Anti-cancer Response of Lonicerae Flos Herbal-acupuncture. The Acupuncture. 2005;22(5):91–7.

22. Lee JM, Choi SW, Cho SH, Rhee SJ. Effect of Forsythia Viridissima Extracts on Antioxidative System and Lipid Peroxidation of Liver in Rats Fed High-Cholesterol Diet. J Nutr Health. 2003;36(10):990–6.

23. Schinella GR, Tournier HA, Prieto JM, Mordujovich D, Rios JL. Antioxidant activity of anti-inflammatory plant extracts. Life Sciences. 2002;70(9):1023–33.
crossref pmid
24. Seo HS. The Experimental Study on Anti-bacterial Potency of Hwangryunhaedok tang Herbal-acupuncture & extract on Staphylococcus aureus & Staphylococcus epidermidis. Journal of Pharmacopuncture. 2006;9(2):99–103.
crossref
25. Kim SM, Choi HS, Seo BI. The Effect of Hwangryunhaedoktang on the Toxicity of Dried Mylabris phalerata Extract. Kor J Herbology. 2010;25(2):41–54.

26. Yang HJ, Joo HA, Baek SC, Park JS, Hong SH. Anti-inflammatory effects of Hwangnyeonhaedok-tang and Fermented Hwangnyeonhaedok-tang. J Korean Med Ophthalmol Otolaryngol Dermatol. 2011;24(2):1–15.

27. Choi JM, Kim HT. Effects of Hwangryunhaedok tang on TNF-α and IL-4 Stimulated TARC, eotaxin, RANTES in the Human Bronchial Epithelial A549 Cells. KJOMPP. 2006;20(6):1649–53.

28. Seol H, Yook TH. Effects of Hwangryunhaedok tang Herbal-acupuncture at G21(Kyonjong) on the Heart Rate Variability. The Acupuncture. 2004;21(6):37–50.

29. Weber A, Knop J, Mauer M. Pattern analysis of human cutaneous mast cell populations by total body surface mapping. BJD. 2003;148(2):224–8.
crossref
30. The Korean society of pathologists. Pathology. 3rd ed. Seoul: Komoonsa. 1997:56:159–60, 169–222.

31. Tierney Lawrence M, McPhee Stephen J, Papadakis Maxine A. Current Medical Diagnosis & Treatment. Seoul: Hanuri. 1999:846–74.

32. Humbett M, Grant JA, Taborda-Barata L, Durham SR, Pfister R, Menz G. High-affinity IgE receptor-bearing cells in bronchial biopsies from atopic and nonatopic asthma. Am J Respir Crit Care Med. 1996;153(6):1931–7.
crossref pmid
33. Marshall JS. Mast-cell responses to pathogens. Nat Rev Immunol. 2004;4(10):787–99.
crossref pmid
34. Abbs AK, Murphy KM, Sher A. Functional diversity of helper T lymphocytes. Nature. 1996;383(6603):787–93.
crossref pmid
35. Borich L. Allergic rhinitis: systemic inflammation and implications for management. J Allergy Clin Immunol. 2003;112(6):1021–31.
crossref pmid
36. Goldsby Richard A, Kindt Thomas J, Osborne Barbara A, Kuby Janis. KUBY Immunology. 5th ed. Seoul: World Science. 2006:104–5, 310327–30.

37. Yun CS, Joo HA, Hwang CY. The Effects of Samulsopungeum and Prednisolone on NC/Nga Atopic Mice. J Korean Med Ophthalmol Otolaryngol Dermatol. 2010;23(1):59–74.

38. Pène J, Rousset F, Brière F, et al. IgE production by normal human lymphocytes is induced by interleukin 4 and suppressed by Interferons gamma and alpha and prostaglandin E2. Proc Natl Acad Sci USA. 1988;85(18):6880–4.
crossref pmid pmc
39. Lee EH, Lee SE, Lee SH. Updates on the diagnosis and treatment of Atopic dermatitis. J Skin Bar Res. 2010;12(1):103–10.



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