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J Acupunct Res > Volume 32(4); 2015 > Article
Jeon, Lee, Kim, and Yim: The Anti-Oxidative Effect of Suhwagije-decoction on the Liver and Spleen Cells of Aged Rats

Abstract

Objectives:

The purpose of this study was to investigate the anti-oxidative effect of Suhwagije-decoction (SGT) pharmacopuncture solution on the liver and spleen cells of aged rats.

Methods:

The liver and spleen cells of 10, 52, and 72 week old Sprague Dawley (SD) rats were divided into three groups: distilled water (DW), Vit.C, and SGT groups, which were treated with DW, Vit.C and SGT pharmacopuncture solution respectively. The levels of superoxide dismutase (SOD), glutathione (GSH) and nitric oxide (NO) were then measured.

Results:

  1. Treatment of SGT pharmacopuncture solution significantly decreased NO concentration in spleen cells of 52 week old rats as compared with that of other groups.

  2. Treatment of SGT pharmacopuncture solution significantly increased SOD activity in spleen cells of 72 week old rats as compared with that of other groups.

Conclusions:

Suhwagije-decoction (SGT) pharmacopuncture solution may have an anti-oxidative effect in the spleen cells of aged rats.

Fig. 1
Effect of aging on the levels of SOD, GSH, and NO in rat liver cells
The levels of SOD, GSH, NO were estimated in liver cells of 10, 52 and 72 weeks old rats. Values represent means±SD of 3 independent experiments.
*: p<0.001 compared to 10w group by Kruskal-wallis test.
: p<0.001 compared to 52w group by Kruskal-wallis test.
acup-32-4-109f1.gif
Fig. 2
Effect of SGT on SOD activity in liver cells of aged rat
Values represent mean±SD of 3 independent experiments.
DW: Cells treated with DW for 48 hours.
Vit.C: Cells treated with vitamin C for 48 hours.
SGT: Cells treated with SGT for 48 hours.
*: p<0.05 compared to DW group by Kruskal-wallis test.
acup-32-4-109f2.gif
Fig. 3
Effect of SGT on GSH concentration in liver cells of aged rat
Values represent mean±SD of 3 independent experiments.
DW: Cells treated with DW for 48 hours.
Vit.C: Cells treated with vitamin C for 48 hours.
SGT: Cells treated with SGT distillate for 48 hours.
*: p<0.05 compared to DW group by Kruskal-wallis test.
acup-32-4-109f3.gif
Fig. 4
Effect of SGT on NO concentration in liver cells of aged rat
Values represent mean±SD of 3 independent experiments.
DW: Cells treated with DW for 48 hours.
Vit.C: Cells treated with vitamin C for 48 hours.
SGT: Cells treated with SGT distillate for 48 hours.
acup-32-4-109f4.gif
Fig. 5
Effect of aging on the levels of SOD, GSH, and NO in rat spleen cells
The levels of SOD, GSH, and NO were estimated in spleen cells of 10, 52 and 72 weeks old rats. Values represent means±SD of 3 independent experiments.
*: p<0.001 compared to 10w group by Kruskal-wallis test.
: p<0.001 compared to 52w group by Kruskal-wallis test.
acup-32-4-109f5.gif
Fig. 6
Effect of SGT on SOD activity in spleen cells of aged rat
Values represent mean±SD of 3 independent experiments.
DW: Cells treated with DW for 48 hours.
Vit.C: Cells treated with vitamin C for 48 hours.
SGT: Cells treated with SGT distillate for 48 hours.
*: p<0.01 compared to DW group by Kruskal-wallis test.
: p<0.01 compared to Vit. C group by Kruskal-wallis test.
acup-32-4-109f6.jpg
Fig. 7
Effect of SGT on GSH concentration in spleen cells of aged rat
Values represent mean±SD of 3 independent experiments.
DW: Cells treated with DW for 48 hours.
Vit.C: Cells treated with vitamin C for 48 hours.
SGT: Cells treated with SGT distillate for 48 hours.
acup-32-4-109f7.jpg
Fig. 8
Effect of SGT on NO concentration in spleen cells of aged rat
Values represent mean±SD of 3 independent experiments.
DW: Cells treated with DW for 48 hours.
Vit.C: Cells treated with vitamin C for 48 hours.
SGT: Cells treated with SGT distillate for 48 hours.
*: p<0.01 compared to DW group by Kruskal-wallis test.
: p<0.01 compared to Vit. C group by Kruskal-wallis test.
acup-32-4-109f8.gif
Table 1
The Compositions of Suhwagije-decoction(SGT)
Herbs Pharmacognostic name Scientific name Dose(g)
SaengJiHwang Rehmanniae Radix Rehmannia glutinosa (Gaertner) Libosch. 4
GeonJiHwang Rehmanniae Radix Siccus Rehmannia glutinosa (Gaertner) Libosch. 4
JiMo Anemarrhenae Rhizoma Anemarrhena asphodeloides Bunge 4
HwangBaek Phellodendri Cortex Phellodendron wilsonii Hayata et Kanehira 4
SanSuYu Corni Fructus Cornus officinalis Sieb. et Zucc. 4
BokBunja Rubi Fructus Rubus coreanus Miq. 4
SiHo Bupleuri Radix Bupleurum falcatum Linne 4
KoSam Sophorae Radix Sophora flavescens Ait. 4
BokRyeong Poria(Hoelen) Poria cocos (Schw.) Wolf 4
Taeksa Alismatis Rhizoma Alisma orientalis (Sam) Juzep 4
YukJongyong Cistanches Herba Cistanche salsa (C. A. Mey) G. Beck 4
Total 44
VI.
VI.

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