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J Acupunct Res > Volume 32(2); 2015 > Article
Kim, Kim, Cho, and Shin: The Effect of Poria Cocos Pharmacopuncture at SP6(Sameumgyo) and ST36(Joksamni) on Chronic Stress Model(CSM) Rats

Abstract

Objectives:

The purpose of this study is evaluating the effect of Poria cocos pharmacopuncture (PCP) at SP6(Sameumgyo) and ST36(Joksamni) on chronic stress model(CSM) rats.

Methods:

The experimental rats were assigned to five groups: normal, CSM(control), PCP1X, PCP2.5X and PCP5X. The experimental rats in CSM(control), PCP1X, PCP2.5X and PCP5X were put through a timetable to cause CSM. PCP(20 μl) was administered at SP6, ST36 to groups PCP1X, PCP2.5X and PCP5X once every three days for a total of six times. The weight of the rats was measured by an electronic scale after PCP injection, once every three days for a total of six times. Blood samples were taken from the rats for analysis of aspartate aminotransferase(AST) and alanine aminotransferase(ALT). Serotonin and norepinephrine were measured by enzyme-linked immunosorbent assay(ELISA) kit.

Results:

The experimental groups(PCP1X, 2.5X, 5X) showed significant body weight increase compared to the control group. Regarding AST and ALT changes in serum, the experimental groups(PCP2.5X, 5X) showed a significant decrease compared to the control group. For Serotonin changes in serum, the experimental group(PCP5X) showed a significant increase compared to the control group. For Norepinephrine changes in serum, the experimental groups(PCP2.5X) showed a significant decrease compared to the control group.

Conclusions:

These data suggest that PCP at SP6, ST36 has therapeutic effects on CSM rats.

Fig. 1.
Effect of Poria cocos pharmacopuncture treatment on the body weight in CSM rats
CSM: chronic stress model.
##: p<0.01 compared with normal. **: p<0.01, *: p<0.05 compared with control.
acupunct-32-2-187f1.gif
Table 1.
Time Table for Chroinc Stress Induction in Rats
Mon Tue Wed Thu Fri
8: 30∼11: 00 LOF & WD TH & Tilt LOF & WD LOF & WD SS
11: 00∼12: 00 NB
12: 00∼13: 00
13: 00∼14: 00 NB NB FS Shaking FS
14: 00∼15: 00
15: 00∼16: 00 FS Shaking SS TH & Tilt TH & Tilt
16: 00∼17: 00
17: 00 0|후 TH TH TH TH TH

LOF: lights on at night. WD: water deprivation. NB: noise bursts. TH: tight husbandary, Tilt, Shaking, FS: forced swimming. SS: stroboscopic light.

Table 2.
Changes on the Body Weight after Poria Cocos Pharmacopuncture Treatment in CSM Rats
Treated times
Groups
CSM Poria cocos pharmacopuncture treatments
Before After 1 2 3 4 5 6
Nor 0.0±0.0 24.7±1.0 35.2 ±1.0 50.0 ±1.2 55.5 ±1.3 71.3±0.9 84.0±1.5 92.8±1.7
Con 0.0±0.0 23.0±1.6 21.5±2.6## 19.4±1.9## 34.9±2.3## 49.8±3.0## 62.8±3.8## 69.8±4.2##
PCP1X 0.0±0.0 17.7±0.8 23.0 ±1.6 33.0±1.4** 32.5±1.5 50.8±1.3 67.2±2.0 71.3±2.3
PCP2.5X 0.0±0.0 18.3±1.4 26.2 ±1.9 37.2±2.4** 41.8±2.3* 53.5±3.2 69.7±3.5 76.3 ±4.0
PCP5X 0.0±0.0 20.2±0.8 26.5 ±1.5 38.5±1.5** 43.2±2.3* 57.0±2.2 72.2±2.3* 79.7 ±3.0

Values are expressed mean±SE. CSM: chronic stress model.

##: p<0.01 compared with normal.

**: p<0.01,

*: p<0.05 compared with control.

Table 3.
Changes on the Aspartate Aminotransferase Contents after Poria Cocos Pharmacopuncture Treatment in CSM Rats
Group Aspartate aminotransferase(U/l)
Nor 76.3±1.7
Con 96.6±7.0
PCP1X 77.5±4.4
PCP2.5X 73.8±1.7
PCP5X 75.0±0.9

Values are expressed mean±SE.

CSM: chronic stress model.

*: p<0.05 compared with control.

Table 4.
Changes on the Alanine Aminotransferase Contents after Poria Cocos Pharmacopuncture Treatment in CSM Rats
Group Alanine aminotransterase(U/l)
Nor 25.5±1.1
Con 31.8±1.9
PCP1X 27.8±1.5
PCP2.5X 25.2±1.4*
PCP5X 22.8±0.8*

Values are expressed mean±SE.

CSM: chronic stress model.

*: p<0.05 compared with control.

Table 5.
Changes on the Serotonin after Poria Cocos Pharmacopuncture Treatment in CSM Rats
Group Serotonin(ng/ml)
Nor 47.9±5.5
Con 29.0±2.1#
PCP1X 39.2±7.8
PCP2.5X 46.7±10.2
PC5X 53.2±8.9*

Values are expressed mean±SE.

CSM: chronic stress model.

#: p<0.05 compared with normal.

*: p<0.05 compared with control.

Table 6.
Changes on the Norepinephrine after Poria Cocos Pharmacopuncture Treatment in CSM Rats
Group Norepinephrine(pg/dl)
Nor 2.86±0.03
Con 2.90±0.04
PCP1X 2.49±0.20
PCP2.5X 2.56±0.08*
PC5X 2.53±0.08

Values are expressed mean±SE.

CSM: chronic stress model.

*: p<0.05 compared with control.

VI.
VI.

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