The Acupuncture 2015; 32(1): 53-66
Published online March 20, 2015
https://doi.org/10.13045/acupunct.2015005
© Korean Acupuncture & Moxibustion Medicine Society
지민정, 임성철, 김재수, 이현종, 이윤규*
대구한의대학교 한의과대학 침구경혈학교실
Correspondence to : *Department of Acupuncture & Moxibustion Medicine, Pohang Oriental Hospital of Daegu Haany University, 411, Saecheonnyeon-daero, Nam-gu, Pohang-si, Gyeongsangbuk-do, 790-826, Republic of Korea
Tel : +82-54-271-8010 E-mail : hanyeesarang@hanmail.net
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objectives : This study was designed to investigate effects of Curculiginis Rhizoma pharmacoacupuncture at ST36 on monosodium iodoacetate-induced osteoarthritic rats.
Methods : Twenty rats were divided into four groups consisting of 5 rats: rats receiving no injection(normal), rats injected with monosodium Iodoacetate(MIA, control), rats injected with MIA and normal saline(N-S), and rats injected with MIA and Curculiginis Rhizoma (CRPA). N-S and CRPA were administered once a day at ST36 during 21 days. After that we examined the weight-bearing ability of hind paws, liver and kidney function, immunocell, cytokines, proteins, and gene expression of cytokines. Injury of synovial tissue was measured by H & E, Safranin O immunofluorescence.
Results : The weight-bearing ability of the hind paws, Serum TNF-α, IL-1β, IL-6, PGE2, LTB4, DPD, Osteocalcin, Protein COX-2 of CRPA decreased significantly. Protein Arachidonate 5 lipoxygenase of CRPA was decreased, but not significantly. Expression of gene COX-2, TNF-α, IL-1β, IL-6, NOS2 of CRPA decreased. In histological observations, CRPA was improved, compared with other control groups.
Conclusions : It can be suggested that Curculiginis Rhizoma pharmaco-acupuncture at ST36 has anti-inflammatory and pain relief effects on monosodium iodoacetate-induced osteoarthritic rats.
Keywords Curculiginis Rhizoma pharmaco-acupuncture; Osteoarthritis; Monosodium iodoacetate(MIA); ST36
Effects of CRPA on changes of weight bearing in hind paws of MIA-induced osteoarthritis rats
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
*: statistically significant compared with control group (
Effects of CRPA on AST, ALT and Creatinine of MIA-induced osteoarthritis rats
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
**: statistically significant compared with control group (
***: statistically significant compared with control group (
Effects of CRPA on WBC, neutronphi and monocyte in the blood of MIA-induced osteoarthritis rats
WBC: white blood cell.
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
*: statistically significant compared with control group (
**: statistically significant compared with control group (
Effects of CRPA on inflammatory cytokines in the serum of MIA-induced osteoarthritis rats
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
*: statistically significant compared with control group(
**: statistically significant compared with control group(
***: statistically significant compared with control group(
Effects of CRPA on COX-2 and Arachidonate 5 lipoxygenase content in synovial tissue of MIA-induced osteoarthritis rats
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
*: statistically significant compared with control group(
Effects of CRPA on expression of inflammatory cytokine contents in synovial tissue of MIA-induced osteoarthritis rats
The results were expressed as mean ± SD(n=2).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
Effects of CRPA on joint pathology(H & E) in joint tissue of MIA-induced osteoarthritis rats
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
Effets of CRPA on joint pathology (safranin O staining) in joint tissue of MIA- induced osteoarthritis rats
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
Effects of CRPA on Body Weight of MIA-induced Osteoarthritis Rats
Group | Body weights(g) | |||
---|---|---|---|---|
0 | 7 | 14 | 21 days | |
Normal | 297.8±4.31 | 333.7±6.94 | 363.5±6.72 | 371.5±4.12 |
Control | 290.8±2.59 | 317.8±4.91 | 345.8±6.27 | 360.0±3.46 |
N-S | 300.0±4.22 | 313.7±4.82 | 336.0±4.95 | 363.6±6.27 |
CRPA | 309.6±5.80 | 312.8±7.99 | 341.2±9.97 | 365.8±10.34 |
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
Effects of CRPA on Changes of Weight Bearing in Hind Paws of MIA-induced Osteoarthritis Rats
Group | ratio | |||
---|---|---|---|---|
0 | 7 | 14 | 21 days | |
Normal | 1.16±0.11 | 1.04±0.05 | 1.05±0.03 | 1.04±0.11 |
Control | 0.61±0.10 | 4.06±1.23 | 2.48±0.20 | 2.60±0.47 |
N-S | 0.86±0.14 | 2.37±0.36 | 1.78±0.25 | 1.80±0.12 |
CRPA | 0.67±0.13 | 1.73±0.36 | 1.57±0.25* | 1.12±0.15* |
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
The Acupuncture 2015; 32(1): 53-66
Published online March 20, 2015 https://doi.org/10.13045/acupunct.2015005
Copyright © Korean Acupuncture & Moxibustion Medicine Society.
지민정, 임성철, 김재수, 이현종, 이윤규*
대구한의대학교 한의과대학 침구경혈학교실
Min Jung Ji, Seong Chul Lim, Jae Soo Kim, Hyun Jong Lee and Yun Kyu Lee*
Department of Acupuncture & Moxibustion, Meridian & Acupoint, College of Oriental Medicine, Deagu Haany University
Correspondence to:*Department of Acupuncture & Moxibustion Medicine, Pohang Oriental Hospital of Daegu Haany University, 411, Saecheonnyeon-daero, Nam-gu, Pohang-si, Gyeongsangbuk-do, 790-826, Republic of Korea
Tel : +82-54-271-8010 E-mail : hanyeesarang@hanmail.net
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objectives : This study was designed to investigate effects of Curculiginis Rhizoma pharmacoacupuncture at ST36 on monosodium iodoacetate-induced osteoarthritic rats.
Methods : Twenty rats were divided into four groups consisting of 5 rats: rats receiving no injection(normal), rats injected with monosodium Iodoacetate(MIA, control), rats injected with MIA and normal saline(N-S), and rats injected with MIA and Curculiginis Rhizoma (CRPA). N-S and CRPA were administered once a day at ST36 during 21 days. After that we examined the weight-bearing ability of hind paws, liver and kidney function, immunocell, cytokines, proteins, and gene expression of cytokines. Injury of synovial tissue was measured by H & E, Safranin O immunofluorescence.
Results : The weight-bearing ability of the hind paws, Serum TNF-α, IL-1β, IL-6, PGE2, LTB4, DPD, Osteocalcin, Protein COX-2 of CRPA decreased significantly. Protein Arachidonate 5 lipoxygenase of CRPA was decreased, but not significantly. Expression of gene COX-2, TNF-α, IL-1β, IL-6, NOS2 of CRPA decreased. In histological observations, CRPA was improved, compared with other control groups.
Conclusions : It can be suggested that Curculiginis Rhizoma pharmaco-acupuncture at ST36 has anti-inflammatory and pain relief effects on monosodium iodoacetate-induced osteoarthritic rats.
Keywords: Curculiginis Rhizoma pharmaco-acupuncture, Osteoarthritis, Monosodium iodoacetate(MIA), ST36
Effects of CRPA on changes of weight bearing in hind paws of MIA-induced osteoarthritis rats
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
*: statistically significant compared with control group (
Effects of CRPA on AST, ALT and Creatinine of MIA-induced osteoarthritis rats
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
**: statistically significant compared with control group (
***: statistically significant compared with control group (
Effects of CRPA on WBC, neutronphi and monocyte in the blood of MIA-induced osteoarthritis rats
WBC: white blood cell.
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
*: statistically significant compared with control group (
**: statistically significant compared with control group (
Effects of CRPA on inflammatory cytokines in the serum of MIA-induced osteoarthritis rats
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
*: statistically significant compared with control group(
**: statistically significant compared with control group(
***: statistically significant compared with control group(
Effects of CRPA on COX-2 and Arachidonate 5 lipoxygenase content in synovial tissue of MIA-induced osteoarthritis rats
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
*: statistically significant compared with control group(
Effects of CRPA on expression of inflammatory cytokine contents in synovial tissue of MIA-induced osteoarthritis rats
The results were expressed as mean ± SD(n=2).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
Effects of CRPA on joint pathology(H & E) in joint tissue of MIA-induced osteoarthritis rats
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
Effets of CRPA on joint pathology (safranin O staining) in joint tissue of MIA- induced osteoarthritis rats
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
Table 1 .. Effects of CRPA on Body Weight of MIA-induced Osteoarthritis Rats.
Group | Body weights(g) | |||
---|---|---|---|---|
0 | 7 | 14 | 21 days | |
Normal | 297.8±4.31 | 333.7±6.94 | 363.5±6.72 | 371.5±4.12 |
Control | 290.8±2.59 | 317.8±4.91 | 345.8±6.27 | 360.0±3.46 |
N-S | 300.0±4.22 | 313.7±4.82 | 336.0±4.95 | 363.6±6.27 |
CRPA | 309.6±5.80 | 312.8±7.99 | 341.2±9.97 | 365.8±10.34 |
The results were expressed as mean ± SD(n=5)..
Normal: rats were no injected with anyone..
Control: rats were injected with MIA..
N-S: rats were injected with MIA and normal saline..
CRPA: rats were injected with MIA and
Table 2 .. Effects of CRPA on Changes of Weight Bearing in Hind Paws of MIA-induced Osteoarthritis Rats.
Group | ratio | |||
---|---|---|---|---|
0 | 7 | 14 | 21 days | |
Normal | 1.16±0.11 | 1.04±0.05 | 1.05±0.03 | 1.04±0.11 |
Control | 0.61±0.10 | 4.06±1.23 | 2.48±0.20 | 2.60±0.47 |
N-S | 0.86±0.14 | 2.37±0.36 | 1.78±0.25 | 1.80±0.12 |
CRPA | 0.67±0.13 | 1.73±0.36 | 1.57±0.25* | 1.12±0.15* |
The results were expressed as mean ± SD(n=5)..
Normal: rats were no injected with anyone..
Control: rats were injected with MIA..
N-S: rats were injected with MIA and normal saline..
CRPA: rats were injected with MIA and
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Effects of CRPA on changes of weight bearing in hind paws of MIA-induced osteoarthritis rats
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
*: statistically significant compared with control group (
Effects of CRPA on AST, ALT and Creatinine of MIA-induced osteoarthritis rats
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
**: statistically significant compared with control group (
***: statistically significant compared with control group (
Effects of CRPA on WBC, neutronphi and monocyte in the blood of MIA-induced osteoarthritis rats
WBC: white blood cell.
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
*: statistically significant compared with control group (
**: statistically significant compared with control group (
Effects of CRPA on inflammatory cytokines in the serum of MIA-induced osteoarthritis rats
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
*: statistically significant compared with control group(
**: statistically significant compared with control group(
***: statistically significant compared with control group(
Effects of CRPA on COX-2 and Arachidonate 5 lipoxygenase content in synovial tissue of MIA-induced osteoarthritis rats
The results were expressed as mean ± SD(n=5).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
*: statistically significant compared with control group(
Effects of CRPA on expression of inflammatory cytokine contents in synovial tissue of MIA-induced osteoarthritis rats
The results were expressed as mean ± SD(n=2).
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
Effects of CRPA on joint pathology(H & E) in joint tissue of MIA-induced osteoarthritis rats
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and
Effets of CRPA on joint pathology (safranin O staining) in joint tissue of MIA- induced osteoarthritis rats
Normal: rats were no injected with anyone.
Control: rats were injected with MIA.
N-S: rats were injected with MIA and normal saline.
CRPA: rats were injected with MIA and